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FluorPen FP 100

FluorPen is a portable, battery-powered fluorometer that enables quick and precise measurement of chlorophyll fluorescence parameters in the laboratory, greenhouse, or in the field. It can be effectively used for studying photosynthetic activity, stress detection, herbicide testing, or mutant screening.

Measured data are sequentially stored in the internal FluorPen memory. Data transfer to a PC is via USB or Bluetooth communication. Comprehensive FluorPen 1.0 software provides data transfer routines and many additional features for data presentation in tables and graphs.

Affordable price and straight-forward two-button operation makes the FluorPen a perfect tool for teaching photosynthesis. Because of its rapid measurement capability and large internal memory, the FluorPen is also an invaluable tool for large plant-screening programs.

The basic model records two parameters:

  • FT - continuous fluorescence yield in non-actinic light. FTis equivalent to F0 if the leaf sample is dark-adapted.
  • QY - Photosystem II quantum yield. QY is equivalent to FV/FM in dark-adapted samples and to FV ' /FM ' in light-adapted samples. FV/FM is the most frequently used chlorophyll fluorescence parameter.
  • FluorPen With the GPS Module FluorPen With a Mini-Computer Antarctic FluorPen
    (1) FluorPen With a GPS Module; (2) FluorPen With a Mini-Computer; (3) Customized FluorPen - Monitoring Pen MP 100

FluorPen FP 100-MAX model includes additional pre-defined protocols for measuring:

  • OJIP Analysis
    Application of chlorophyll fluorescence fast-transient analysis (OJIP) is a simple and non-invasive tool to monitor chloroplast function. Provided OJIP analysis is used as sensitive and reliable fast test for the functionality and vitality of photosynthetic system.
  • NPQ - Non-Photochemical Quenching
    Provided are two predefined NPQ protocols differing in the duration of light exposure and dark recovery phase as well as in the number of intervals between the pulses. It is typically used for quantification of photochemical and non-photochemical quenching in dark-adapted samples.
  • Light Curve
    There are three predefined Light Curve protocols based on pulse modulated fluorometry differing in number and duration of single light phases and light intensities. Light Curve protocols provide successive measurements of the sample photosynthesis under various light intensities of continuous illumination relating the rate of photosynthesis to photon flux density.
  • Photosynthesis research
  • Photosynthesis education
  • Plant & molecular biology
  • Agriculture
  • Large plant-screening programs
  • Biotechnology
  • FluorPen in the Field Experiment
    FluorPen in the Field Experiment
  • Different leaf-clips for gentle but firm sample holding:
  • Standard leaf-clip: suitable for experiments where short term dark adaptation is needed
    Standard Leaf-Clip
  • Open leaf-clip: suitable for measurements in ambient light (experiments where no dark adaptation is required)
    Open Leaf-Clip
  • Customized sample holders: for instance, for mosses, lichens and other experimental material
    Open Leaf-Clip
  • Detachable leaf-clips: suitable for experiments where long term dark adaptation is needed
    Open Leaf-Clip

    Open Leaf-Clip
  • Two predefined NPQ protocols differing in the duration of light exposure and dark recovery phase as well as in the number of intervals between the pulses
  • Typically used for quantification of photochemical and non-photochemical quenching in dark-adapted samples
  • NPQ 1 protocol: light duration 60s, 5 pulses; dark recovery duration 88s, 3 pulses
  • NPQ 2 protocol: light duration 200s, 10 pulses; dark recovery duration 390s, 7 pulses
  • NPQ Protocol Visualization
    NPQ Protocol Visualization
  • Three predefined protocols differing in number and duration of single light phases and light intensities
  • Based on pulse modulated fluorometry
  • Successive measurments of the sample photosynthesis under various light intensities of continuous illumination
  • Light response curve relating the rate of photosynthesis to photon flux density
  • Light Curve Protocol Visualization
    Light Curve Protocol Visualization
  • Bckg = background
  • F0: = F50µs; fluorescence intensity at 50 µs
  • FJ: = fluorescence intensity at j-step (at 2 ms)
  • Fi: = fluorescence intensity at i-step (at 60 ms)
  • FM: = maximal fluorescence intensity
  • FV: = FM - F0 (maximal variable fluorescence)
  • VJ = (FJ - F0) / (FM - F0)
  • Vi = (Fi - F0) / (FM - F0)
  • FM / F0
  • FV / F0
  • FV/ FM
  • M0 or (dV / dt)0 = TR0 / RC - ET0 / RC = 4 (F300 - F0) / (FM - F0)
  • Area = area between fluorescence curve and FM (background subtracted)
  • Fix Area = total area above the OJIP fluorescence transient - between F40µ and F1s(background subtracted)
  • SM = area / FM - F0 (multiple turn-over)
  • Ss = the smallest Sm turn-over (single turn-over)
  • N = SM . M0 . (1 / VJ) turn-over number QA
  • Phi_P0 = 1 - (F0 / FM (or FV / FM)
  • Psi_0 = 1 - VJ
  • Phi_E0 = (1 - F0 / FM)) . Psi_0
  • Phi_D0 = 1 - Phi_P0 - (F0 / FM)
  • Phi_Pav = Phi_P0 - (SM / tFM); tFM) = Time to reach FM (in ms)
  • ABS / RC = M0 . (1 / VJ) . (1 / Phi_P0)
  • TR0 / RC = M0 . (1 / VJ)
  • ET0 / RC = M0 . (1 / VJ) . Phi_0)
  • DI0 / RC = (ABS / RC) - (TR0 / RC)
  • OJIP Curve
Formulas Derived From:
R.J. Strasser, A. Srivastava and M. Tsimilli-Michael (2000): The fluorescence transient as a tool to characterize and screen photosynthetic samples. In: Probing Photosynthesis: Mechanism, Regulation and Adaptation (M. Yunus, U. Pathre and P. Mohanty, eds.), Taylor and Francis, UK, Chapter 25, pp 445-483.
  • FluorPen 1.0 software (Windows 2000, XP, or higher compatible*)
  • Bluetooth, USB or serial communication
  • Real-time and remote control functions
  • GPS mapping plug-in
  • Export to Microsoft Excel
  • FluorPen Software Window: OJIP Curves With Data Sheet
    FluorPen Software Window: OJIP Curves With Data Sheet
* Windows is a registered trademark of Microsoft Corporation
  • Measured/Calculated Parameters:
    F0 , FT , FM , FM ' , QY, OJIP*, NPQ 1,2*, and Light Curve 1,2,3*
  • Saturating Light:
    Adjustable from 0 to 100 % (up to 3,000 µmol(photon).m-2.s-1
  • Actinic Light:
    Adjustable from 0 to 100 % (up to 1,000 µmol(photon).m-2.s-1
  • Measuring Light:
    Adjustable from 0 to 100 % (up to 0.09 µmol(photon).m-2 per pulse)
  • Detector Wavelength Range:
    PIN photodiode with 697 to 750 nm bandpass filters
  • FluorPen 1.0 Software:
    Windows 2000, XP, or higher**
  • Memory Capacity:
    Up to 4 Mb
  • Internal Data Logging:
    Up to 100,000 data points
  • Display:
    2 x 8 characters LC display
  • Keypad:
    Sealed, 2-key tactile response
  • Keypad Escape Time:
    Turns off after 5 minutes of no use
  • Power Supply:
    4 AAA alkaline or rechargeable batteries
  • Battery Life:
    48 hours typical with full operation
  • Low Battery Detection:
    Low battery indication displayed
  • Size:
    120 mm x 57 mm x 30 mm 4.7" x 2.2" x 1.2"
  • Weight:
    180 g, 6.5 oz
  • Sample Holder:
    Mechanical leaf clip - standard, open, or detachable
  • Operating Conditions:
    Temperature: 0 to 55 ºC; 32 to 130 ºF Relative humidity: 0 to 95 % (non-condensing)
  • Storage Conditions:
    Temperature: -10 to +60 ºC; 14 to +140 ºF Relative humidity: 0 to 95 % (non-condensing)
  • Warranty:
    1 year parts and labor
* Only in the FluorPen FP 100-MAX or the FluorPen models including protocol update
** Windows is a registered trademark of Microsoft Corporation
  • Obukola Ajigboye et al. (2014): Pesticide Biochemistry and Physiology 114: 52-60.
  • Ptushenko V.V. et al. (2013): Biosystems 114 (2): 85-97.
  • Samoilova O.P. et al. (2011): Biosystems 105 (1): 41-48.
  • Frolec J. et al. (2010): Plant Cell Rep. 29: 705–714.
  • Cessna S. et al. (2010): J. Nat. Res. & Life Scence Education 39: 22-30.
  • Ruiz-Sanchez M. et al. (2010): J. Physiol. 167 (11): 862-869.
  • Fernandez-Marin Becerril J.M. et al. (2010): Planta 231: 1335–1342.
  • Harding S.A. et al. (2009): J. Exp. Botany 60 (12): 3443-3452.
  • Kuvykin I.V. et al. (2009): Cell Biophysics 54 (4): 455-464.
  • Macek P. et al. (2009): J. Exp. Botany 60 (12): Acta Oecologica 35: 778–785.
  • Rosescu M.R. et al. (2009): Annals, Food Science and technology 10: 115-119.
  • Zhang M. et al. (2009): Ecology and Environmemtal Sciences 18 (6): 2272-2277.
  • Woo N.S. et al. (2009): Plant methods 4 (27).
  • Klem K. and Bajerova E. (2008): AgEng 2008 Conference, Hersonissos, Crete.

Pricing

  • Orders and Payments
  • FluorPen FP 100-MAX/USB
    1.999,- €
  • FluorPen FP 100-MAX/BT
    2.149,- €
  • FluorPen FP 100-MAX-D/USB
    2.199,- €
  • FluorPen FP 100-MAX-D/BT
    2.349,- €
  • FluorPen FP 100-MAX-W/USB
    2.099,- €
  • FluorPen FP 100-MAX-W/BT
    2.249,- €
  • FluorPen FP 100
    999,- €
  • FluorPen FP 100/BT
    1.649,- €
  • FluorPen FP 100/USB
    1.499,- €
  • Detachable Leaf-Clips *
    140,- €
  • Mini-Computer *
    390,- €
  • Spare USB Communication Cable *
    39,- €
  • GPS Module *
    249,- €
  • Integrated Light Meter
    290,- €
  • Battery Charger *
    49,- €
  • Protocol Update
    579,- €

FluorPen Versions

FluorPen FP 100-MAX
Includes one of the communication modules (Bluetooth or USB), FluorPen 1.0 software, protocol update, user's guide. Measures Ft, QY, NPQ, OJIP, and Light Curve. Equipped with a standard leaf-clip.


FluorPen FP 100-MAX-W
This version of the FluorPen FP 100-MAX is equipped with a special open leaf-clip that enables measurements in ambient light. The open leaf-clip may is supplied with a cover for short term dark adaptation of measured samples.


FluorPen FP 100-MAX-D
Includes one of the communication modules (Bluetooth or USB), FluorPen 1.0 software, protocol update, user's guide. Measures Ft, QY, NPQ, OJIP, and Light Curve. Adapted for use with detachable leaf-clips; leaf clips sold separately.


FluorPen FP 100
Standard FluorPen version with no PC communication. Data are saved to the device internal memory only.


FluorPen FP 100-B
Standard FluorPen version supplemented with Bluetooth communication module and FluorPen 1.0 software for data transfer to a PC. Measured parameters: FT and QY.


FluorPen FP 100-U
Standard FluorPen version supplemented with USB communication module and FluorPen 1.0 software for data transfer to a PC. Measured parameters: FT and QY.


Optional Features and Accessories

Detachable Leaf-Clips
Supplementary detachable leaf-clips for use with the FluorPen FP 100-MAX-D. 10-piece set.


Mini-Computer
Small, portable notebook: Intel Celeron two-core procesor; 10.1“ multi-touch HD, 2 GB operation memory, 500 GB hard disc capacity; WiFi; BT; Windows 8 pre-installed; HDMI; web camera.


Spare USB Communication Cable
Spare USB cable allowing connection between the "pen" devices and the PC (the "pen" device is delivered with one USB cable, which is included in the device price).


Protocol Update
Enhancement to the FluorPen FP 100 version: Enables analysis of fluorescence induction curves and related fluorescence parameters - OJIP, NPQ, and Light Curve.


GPS Module
To record the exact location of the measurements performed by the pen-category instruments.


Battery Charger
Includes battery charger and 4 AAA alkaline rechargeable batteries. EU norm only.

Download

Software
FluorPen 1.0
OS: Win2000/XP/Win7 (32bit)/Win 8 (32 bit)
Language: English
Size: 2.2MB


Documents
FluorPen Series Manual
Type: PDF
Language: English
Size: 4.9MB


FluorPen Explain
Type: PDF
Language: English
Size: 2.9MB


SpectraPen Manual
Type: PDF
Language: English
Size: 460kB


PolyPen Manual
Type: PDF
Language: English
Size: 580kB


AquaPen Manual
Type: PDF
Language: English
Size: 3.9MB


AquaPen Manual (Czech)
Type: PDF
Language: Czech
Size: 4.2MB


N-Pen Brief Guide
Type: PDF
Language: English
Size: 106kB


PlantPen NDVI-PRI Operation Manual
Type: PDF
Language: English
Size: 1.9MB


FluorPen Teaching Material

Kindly provided by Chris Chastain from the Minnesota State University in Moorhead.

FluorPen Explain (PDF file, 2,9 MB)

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