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FluorPen FP 100

FluorPen is a portable, battery-powered fluorometer that enables quick and precise measurement of chlorophyll fluorescence parameters in the laboratory, greenhouse, or in the field. It can be effectively used for studying photosynthetic activity, stress detection, herbicide testing, or mutant screening.

Measured data are sequentially stored in the internal FluorPen memory. Data transfer to a PC is via USB or Bluetooth communication. Comprehensive FluorPen 1.0 software provides data transfer routines and many additional features for data presentation in tables and graphs.

Affordable price and straight-forward two-button operation makes the FluorPen a perfect tool for teaching photosynthesis. Because of its rapid measurement capability and large internal memory, the FluorPen is also an invaluable tool for large plant-screening programs.

The basic model records two parameters:

  • FT - continuous fluorescence yield in non-actinic light. FTis equivalent to F0 if the leaf sample is dark-adapted.
  • QY - Photosystem II quantum yield. QY is equivalent to FV/FM in dark-adapted samples and to FV ' /FM ' in light-adapted samples. FV/FM is the most frequently used chlorophyll fluorescence parameter.
  • FluorPen With the GPS Module FluorPen With a Mini-Computer Antarctic FluorPen
    (1) FluorPen With a GPS Module; (2) FluorPen With a Mini-Computer; (3) Customized FluorPen - Monitoring Pen MP 100

FluorPen FP 100-MAX model includes additional pre-defined protocols for measuring:

  • OJIP Analysis
    Application of chlorophyll fluorescence fast-transient analysis (OJIP) is a simple and non-invasive tool to monitor chloroplast function. Provided OJIP analysis is used as sensitive and reliable fast test for the functionality and vitality of photosynthetic system.
  • NPQ - Non-Photochemical Quenching
    Provided are two predefined NPQ protocols differing in the duration of light exposure and dark recovery phase as well as in the number of intervals between the pulses. It is typically used for quantification of photochemical and non-photochemical quenching in dark-adapted samples.
  • Light Curve
    There are three predefined Light Curve protocols based on pulse modulated fluorometry differing in number and duration of single light phases and light intensities. Light Curve protocols provide successive measurements of the sample photosynthesis under various light intensities of continuous illumination relating the rate of photosynthesis to photon flux density.
  • Photosynthesis research
  • Photosynthesis education
  • Plant & molecular biology
  • Agriculture
  • Large plant-screening programs
  • Biotechnology
  • FluorPen in the Field Experiment
    FluorPen in the Field Experiment
  • Different leaf-clips for gentle but firm sample holding:
  • Standard leaf-clip: Suitable for experiments where short term dark adaptation is needed
    Standard Leaf-Clip
  • Open leaf-clip: Suitable for measurements in ambient light (experiments where no dark adaptation is required)
    Open Leaf-Clip
  • Customized sample holders: For instance, for mosses, lichens and other experimental material
    Open Leaf-Clip
  • Detachable leaf-clips: Suitable for experiments where long term dark adaptation is needed. They also allow simultaneous measurings on numerous plants. Clips are then positioned on different plants and readings can be saved one after another by attaching the device unit to each clip. Construction of the clip allows measuring in ambient light, too (see the bottom photo).

    Detachable Leaf-Clip

    Detachable Leaf-Clip in Field

    Detachable Leaf-Clip Explain
  • Two predefined NPQ protocols differing in the duration of light exposure and dark recovery phase as well as in the number of intervals between the pulses
  • Typically used for quantification of photochemical and non-photochemical quenching in dark-adapted samples
  • NPQ 1 protocol: light duration 60s, 5 pulses; dark recovery duration 88s, 3 pulses
  • NPQ 2 protocol: light duration 200s, 10 pulses; dark recovery duration 390s, 7 pulses
  • NPQ Protocol Visualization
    NPQ Protocol Visualization
  • Three predefined protocols differing in number and duration of single light phases and light intensities
  • Based on pulse modulated fluorometry
  • Successive measurments of the sample photosynthesis under various light intensities of continuous illumination
  • Light response curve relating the rate of photosynthesis to photon flux density
  • Light Curve Protocol Visualization
    Light Curve Protocol Visualization
  • Bckg = background
  • F0: = F50µs; fluorescence intensity at 50 µs
  • FJ: = fluorescence intensity at j-step (at 2 ms)
  • Fi: = fluorescence intensity at i-step (at 60 ms)
  • FM: = maximal fluorescence intensity
  • FV: = FM - F0 (maximal variable fluorescence)
  • VJ = (FJ - F0) / (FM - F0)
  • Vi = (Fi - F0) / (FM - F0)
  • FM / F0
  • FV / F0
  • FV/ FM
  • M0 or (dV / dt)0 = TR0 / RC - ET0 / RC = 4 (F300 - F0) / (FM - F0)
  • Area = area between fluorescence curve and FM (background subtracted)
  • Fix Area = total area above the OJIP fluorescence transient - between F40µ and F1s(background subtracted)
  • SM = area / FM - F0 (multiple turn-over)
  • Ss = the smallest Sm turn-over (single turn-over)
  • N = SM . M0 . (1 / VJ) turn-over number QA
  • Phi_P0 = 1 - (F0 / FM (or FV / FM)
  • Psi_0 = 1 - VJ
  • Phi_E0 = (1 - F0 / FM)) . Psi_0
  • Phi_D0 = 1 - Phi_P0 - (F0 / FM)
  • Phi_Pav = Phi_P0 - (SM / tFM); tFM) = Time to reach FM (in ms)
  • ABS / RC = M0 . (1 / VJ) . (1 / Phi_P0)
  • TR0 / RC = M0 . (1 / VJ)
  • ET0 / RC = M0 . (1 / VJ) . Phi_0)
  • DI0 / RC = (ABS / RC) - (TR0 / RC)
  • OJIP Curve
Formulas Derived From:
R.J. Strasser, A. Srivastava and M. Tsimilli-Michael (2000): The fluorescence transient as a tool to characterize and screen photosynthetic samples. In: Probing Photosynthesis: Mechanism, Regulation and Adaptation (M. Yunus, U. Pathre and P. Mohanty, eds.), Taylor and Francis, UK, Chapter 25, pp 445-483.
  • FluorPen 1.0 software (Windows 2000, XP, or higher compatible*)
  • Bluetooth, USB or serial communication
  • Real-time and remote control functions
  • GPS mapping plug-in
  • Export to Microsoft Excel
  • FluorPen Software Window: OJIP Curves With Data Sheet
    FluorPen Software Window: OJIP Curves With Data Sheet
* Windows is a registered trademark of Microsoft Corporation
  • Measured/Calculated Parameters:
    F0 , FT , FM , FM ' , QY, OJIP*, NPQ 1,2*, and Light Curve 1,2,3*
  • Saturating Light:
    Adjustable from 0 to 100 % (up to 3,000 µmol(photon).m-2.s-1
  • Actinic Light:
    Adjustable from 0 to 100 % (up to 1,000 µmol(photon).m-2.s-1
  • Measuring Light:
    Adjustable from 0 to 100 % (up to 0.09 µmol(photon).m-2 per pulse)
  • Emitter:
    Blue 470 nm LED - optically filtered and precisely focused
  • Detector Wavelength Range:
    PIN photodiode with 697 to 750 nm bandpass filters
  • FluorPen 1.0 Software:
    Windows 2000, XP, or higher**
  • Memory Capacity (4 Mb):
    Ft: up to 149,000 measurings
    QY: up to 95,000 measurings
    LC1: up to 3,000 measurings
    LC2: up to 3,500 measurings
    LC3: up to 2,600 measurings
    NPQ1: up to 800 measurings
    NPQ2: up to 500 measurings
    OJIP: up to 1,100 measurings
  • Internal Data Logging:
    Up to 100,000 data points
  • Display:
    2 x 8 characters LC display
  • Keypad:
    Sealed, 2-key tactile response
  • Keypad Escape Time:
    Turns off after 5 minutes of no use
  • Power Supply:
    4 AAA alkaline or rechargeable batteries
  • Battery Life:
    48 hours typical with full operation
  • Low Battery Detection:
    Low battery indication displayed
  • Size:
    120 mm x 57 mm x 30 mm 4.7" x 2.2" x 1.2"
  • Weight:
    180 g, 6.5 oz
  • Sample Holder:
    Mechanical leaf clip - standard, open, or detachable
  • Operating Conditions:
    Temperature: 0 to 55 ºC; 32 to 130 ºF Relative humidity: 0 to 95 % (non-condensing)
  • Storage Conditions:
    Temperature: -10 to +60 ºC; 14 to +140 ºF Relative humidity: 0 to 95 % (non-condensing)
  • Warranty:
    1 year parts and labor
* Only in the FluorPen FP 100-MAX or the FluorPen models including protocol update
** Windows is a registered trademark of Microsoft Corporation
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  • AROCA R., RUIZ-LOZANO M. J., ZAMARREÑO A. M., ET AL. (2013). Arbuscular mycorrhizal symbiosis influences strigolactone production under salinity and alleviates salt stress in lettuce plants. Journal of Plant Physiology, Volume 170, Issue 1, Pages 47-55. DOI:10.1016/j.jplph.2012.08.020
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  • PTUSHENKO V.V., PTUSHENKO E. A., SAMOILOVA O. P. ET AL. (2013). Chlorophyll fluorescence in the leaves of TRADESCANTIA species of different ecological groups: Induction events at different intensities of actinic light. Biosystems. Volume 114, Issue 2, Pages 85–97. DOI:10.1016/j.biosystems.2013.08.001
  • SHAKHATREH Y., CARVALHO P., FOULKES J. ET AL. (2013). ACLIMAS annual meeting – Rabat, 23 October 2013. http://www.aclimas.eu/Reports-Document/ACLIMAS%20SWIM-DP%202nd%20Annual%20Meeting,%20Rabat,%2023-24%20October%202013/ACLIMAS,%20Jordan%20achievements%20and%20future%20plans.pdf
  • VREDENBERG W. AND PAVLOVIČ A. (2012). Chlorophyll a fluorescence induction (Kautsky curve) in a Venus flytrap (Dionaea muscipula) leaf after mechanical trigger hair irritation. Journal of Plant Physiology. Volume 170, Pages 242-250. DOI:10.1016/j.jplph.2012.09.009
  • KLEM K., AČ A., HOLUB P. ET AL. (2012). Interactive effects of PAR and UV radiation on the physiology, morphology and leaf optical properties of two barley varieties. Environmental and Experimental Botany. Volume 75, Pages 52-64. DOI:10.1016/j.envexpbot.2011.08.008, http://www.sciencedirect.com/science/article/pii/S0098847211001900
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  • COWLEY R. AND LUCKETT D. (2011) Chlorophyll fluorescence as a method to detect moisture-limiting stress in canola. 17thAustralian Research Assembly on Brassicas (ARAB)
  • KOCUREK V., VONDRA M. AND SMUTNÝ, V. (2011). Efficacy of reduced doses of bentazone assessed by instruments based on measurement of chlorophyll fluorescence. Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis. Volume 59, Pages 137-144. DOI: 10.11118/actaun201159010137
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  • Orders and Payments
  • FluorPen FP 100-MAX/USB
    1.999,- €
  • FluorPen FP 100-MAX/BT
    2.149,- €
  • FluorPen FP 100-MAX-D/USB
    2.199,- €
  • FluorPen FP 100-MAX-D/BT
    2.349,- €
  • FluorPen FP 100-MAX-W/USB
    2.099,- €
  • FluorPen FP 100-MAX-W/BT
    2.249,- €
  • FluorPen FP 100
    999,- €
  • FluorPen FP 100/BT
    1.649,- €
  • FluorPen FP 100/USB
    1.499,- €
  • Detachable Leaf-Clips *
    140,- €
  • Mini-Computer *
    390,- €
  • Spare USB Communication Cable *
    39,- €
  • GPS Module *
    249,- €
  • Integrated Light Meter
    290,- €
  • Battery Charger *
    49,- €
  • Protocol Update
    579,- €

FluorPen Versions

FluorPen FP 100-MAX
Includes one of the communication modules (Bluetooth or USB), FluorPen 1.0 software, protocol update, user's guide. Measures Ft, QY, NPQ, OJIP, and Light Curve. Equipped with a standard leaf-clip.

FluorPen FP 100-MAX-W
This version of the FluorPen FP 100-MAX is equipped with a special open leaf-clip that enables measurements in ambient light. The open leaf-clip may is supplied with a cover for short term dark adaptation of measured samples.

FluorPen FP 100-MAX-D
Includes one of the communication modules (Bluetooth or USB), FluorPen 1.0 software, protocol update, user's guide. Measures Ft, QY, NPQ, OJIP, and Light Curve. Adapted for use with detachable leaf-clips; leaf clips sold separately.

FluorPen FP 100
Standard FluorPen version with no PC communication. Data are saved to the device internal memory only.

FluorPen FP 100-B
Standard FluorPen version supplemented with Bluetooth communication module and FluorPen 1.0 software for data transfer to a PC. Measured parameters: FT and QY.

FluorPen FP 100-U
Standard FluorPen version supplemented with USB communication module and FluorPen 1.0 software for data transfer to a PC. Measured parameters: FT and QY.

Optional Features and Accessories

Detachable Leaf-Clips
Supplementary detachable leaf-clips for use with the FluorPen FP 100-MAX-D. 10-piece set.

Small, portable notebook: Intel Celeron two-core procesor; 10.1“ multi-touch HD, 2 GB operation memory, 500 GB hard disc capacity; WiFi; BT; Windows 8 pre-installed; HDMI; web camera.

Spare USB Communication Cable
Spare USB cable allowing connection between the "pen" devices and the PC (the "pen" device is delivered with one USB cable, which is included in the device price).

Protocol Update
Enhancement to the FluorPen FP 100 version: Enables analysis of fluorescence induction curves and related fluorescence parameters - OJIP, NPQ, and Light Curve.

GPS Module
To record the exact location of the measurements performed by the pen-category instruments.

Battery Charger
Includes battery charger and 4 AAA alkaline rechargeable batteries. EU norm only.


FluorPen 1.0
OS: Windows 2000 / Windows XP / Windows 7 (32bit, 64bit) / Windows 8 (32 bit, 64bit)/ Windows 10 (32 bit, 64bit),
Language: English
Size: 11.1 MB

SpectraPen_PolyPen 1.0
OS: Windows 7 (32bit, 64bit) and higher
Language: English
Size: 902 KB

FluorPen Series Manual
Type: PDF
Language: English
Size: 4.7 MB

FluorPen - List of References
Type: PDF
Language: Czech
Size: 779 KB

FluorPen Explain
Type: PDF
Language: English
Size: 2.8 MB

SpectraPen SP 100 Manual
Type: PDF
Language: English
Size: 1.4 MB

SpectraPen SP 100 - List of References
Type: PDF
Language: Czech
Size: 131 KB

SpectraPen LM 500 Manual
Type: PDF
Language: English
Size: 2.9 MB

PolyPen Manual
Type: PDF
Language: English
Size: 2.2 MB

PolyPen - List of References
Type: PDF
Language: Czech
Size: 148 KB

AquaPen Manual
Type: PDF
Language: English
Size: 3.9 MB

AquaPen Manual (Czech)
Type: PDF
Language: Czech
Size: 4.0 MB

AquaPen - List of References
Type: PDF
Language: Czech
Size: 1.1 MB

LaiPen Manual
Type: PDF
Language: English
Size: 3.3 MB

LaiPen - List of References
Type: PDF
Language: Czech
Size: 135 KB

N-Pen Brief Guide
Type: PDF
Language: English
Size: 104 KB

N-Pen - List of References
Type: PDF
Language: Czech

PlantPen NDVI-PRI Operation Manual
Type: PDF
Language: English
Size: 3.3 MB

PlantPen - List of References
Type: PDF
Language: Czech
Size: 181 KB

FluorPen Teaching Material

Kindly provided by Chris Chastain from the Minnesota State University in Moorhead.

FluorPen Explain (PDF file, 2,9 MB)

Other Pocket-Sized-Instruments

FluorPen FP 100

FluorPen is a hand-held fluorometer that measures chlorophyll fluorescence parameters in a fully automated and rapid manner.
- Photosynthesis Research & Education
- Large plant screening programs
- Agriculture & Forestry
- Biotechnology

PAR-FluorPen FP 100-MAX-LM

PAR-FluorPen is used to measure fluorescence and Photosynthetic Photon Flux Density (PPFD) of photosynthetically active radiation (PAR).
- Photosynthesis Research & Education
- Plant & Molecular Biology
- Agriculture & Forestry
- Biotechnology

Monitoring Pen MP 100

Monitoring Pen is a robust and weather-proof version of the popular FluorPen that is intended for unattended use in a field.
- Photosynthesis Research & Education
- Long-Term, Autonomous Fluorometry Experiments

AquaPen-C AP-C 100

AquaPen-C is a new cuvette version of the popular FluorPen fluorometer. It is optimized for measuring chlorophyll fluorescence in green algae and cyanobacteria. AquaPen sensitivity 500 ng Chl/l enables measuring in natural water.
- Photosynthesis Research & Education
- Biotechnology
- Limnology & Oceanography

AquaPen-P AP-P 100

AquaPen-P is equipped with a submersible optical probe. It suits investigations in algal suspensions and natural waters. AquaPen sensitivity: 500 ng Chl/l.
- Photosynthesis Research & Education
- Phycology
- Limnology & Oceanography
- Biotechnology

SpectraPen LM 500

SpectraPen with precise radiometric calibration and integrated cosine corrector. Number of formulas and calculated parameters: PAR, Watt, Lux, Lumen, CIE color coordinates, color rendering indexes, color temperatures.
- Light Characteristics Measurements
- Light Source Testing and Quality Control
- Color Measurement

SpectraPen SP 100

SpectraPen is a handheld, programmable spectrophotometer that provides instant readings and spectral lines on interactive touch screen.
- Visible Light Source Testing
- Color Measurement
- Chemical Measurement

PolyPen RP 400

PolyPen RP 400 is a complete system for measurement of spectral reflectance on flat leaves as well as measurements of transmittance and absorbance of any external light source. Integrated software is capable of automatic calculation of all common vegetation indices.

PlantPen PRI 200 & NDVI 300

PlantPen is a low-cost, handheld device that characterizes plants by means of reflectance. The two standard versions measure PRI and NDVI.
- Photosynthesis Research & Education
- Vegetation Productivity
- Vegetation Stress Studies
- Agriculture

N-Pen N 100

N-Pen is a light-weight, battery-powered instrument that provides quick measurement of actual nitrogen content in plants throughout their growing season.
- Significant Yield Increase
- Quick Tuning of Nitrogen Management in Crops
- Saving Nitrogen Application Costs

LaiPen LP 100

LaiPen is a light-weight, battery-powered device that mesures Leaf Area Index (LAI) and Photosynthetically Active Radiation (PAR). It provides fast and easily repeatable measurements with instant readouts that can be exported to a PC for further processing.
- Studies of Canopy Productivity
- Studies of Forest Dynamism
- Remote Sensing

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