AquaPen-C AP-C 100

AquaPen-C AquaPen-C is a new cuvette version of the FluorPen fluorometer. It has blue and red measuring lights that enable measuring photosynthetic parameters both in algal and cyanobacterial suspensions.

Due to its ultra-high sensitivity - up to 10 ng Chl/l, the AquaPen-C can measure natural water samples containing low concentrations of phytoplankton.

Incorporated Bluetooth/USB/serial communication technology and the FluorPen 1.0 software provide visualization and data transfer routines to a PC.

Applications:

  • Photosynthesis research
  • Photosynthesis education
  • Phycology
  • Limnology
  • Oceanography
  • Biotechnology

AquaPen-C Measures:

  • FT - Instantaneous Chlorophyll Fluorescence. FT is equivalent to F0 if the sample is dark-adapted.
  • QY - Quantum Yield. QY is a measure of the Photosystem II efficiency. QY is equivalent to FV/FM in dark-adapted samples and to FV ' /FM ' in light-adapted samples.
  • OJIP - Chlorophyll Fluorescence Transient. OJIP measurement is used as an important biophysical signal that reflects the time course of photosynthesis.
  • NPQ - Non-Photochemical Quenching. NPQ indicates thermal dissipation of absorbed light energy during photosynthesis.
  • LC - Light Curve. Photosystem II Quantum Yield estimated from fluorescence that is measured sequentially in several different light levels.
  • OD - Optical Density at 680 nm and 720 nm.

NPQ Protocol
NPQ Protocol Includes Five Measurements in Actinic Light
and Three Measurements During Dark Relaxation.
NPQ_Ln = (FM - FM_Ln) / FM_Ln
NPQ_Lss = (FM - FM_Lss) / FM_Lss
NPQ_Dn = (FM – FM_Dn) / FM_Dn

Light Curve Protocol
Adaptation of QY to Six Different Light Levels

Software:

  • FluorPen 1.0 software (Windows 2000, XP, or higher compatible*)
  • Bluetooth, USB or serial communication
  • Real-time and remote control functions
  • Export to Microsoft Excel
  • GPS mapping plug-in

Calculated parameters
FluorPen Software Window: OJIP Curves With Data Sheet

Explanation of OJIP Parameters:

  • Bckg = background
  • F0: = F50µs; fluorescence intensity at 50 µs
  • FJ: = fluorescence intensity at j-step (at 2 ms)
  • Fi: = fluorescence intensity at i-step (at 60 ms)
  • FM: = maximal fluorescence intensity
  • FV: = FM - F0 (maximal variable fluorescence)
  • VJ = (FJ - F0) / (FM - F0)
  • Vi = (Fi - F0) / (FM - F0)
  • FM / F0
  • FV / F0
  • FV/ FM
  • M0 or (dV / dt)0 = TR0 / RC - ET0 / RC = 4 (F300 - F0) / (FM - F0)
  • Area = area between fluorescence curve and FM (background subtracted)
  • Fix Area = total area above the OJIP fluorescence transient - between F40µ and F1s(background subtracted)
  • SM = area / FM - F0 (multiple turn-over)
  • Ss = the smallest Sm turn-over (single turn-over)
  • N = SM . M0 . (1 / VJ) turn-over number QA
  • Phi_P0 = 1 - (F0 / FM (or FV / FM)
  • Psi_0 = 1 - VJ
  • Phi_E0 = (1 - F0 / FM)) . Psi_0
  • Phi_D0 = 1 - Phi_P0 - (F0 / FM)
  • Phi_Pav = Phi_P0 - (SM / tFM); tFM) = Time to reach FM (in ms)
  • ABS / RC = M0 . (1 / VJ) . (1 / Phi_P0)
  • TR0 / RC = M0 . (1 / VJ)
  • ET0 / RC = M0 . (1 / VJ) . Phi_0)
  • DI0 / RC = (ABS / RC) - (TR0 / RC)

Formulas Derived From:
R.J. Strasser, A. Srivastava and M. Tsimilli-Michael (2000): The fluorescence transient as a tool to characterize and screen photosynthetic samples. In: Probing Photosynthesis: Mechanism, Regulation and Adaptation (M. Yunus, U. Pathre and P. Mohanty, eds.), Taylor and Francis, UK, Chapter 25, pp 445-483.



Transport Case

Technical Specification:

  • Measured/Calculated Parameters:
    F0 , FT , FM , FM ' , QY, OJIP, NPQ, LC 1, LC 2, OD 680 , OD 720
  • Actinic and Saturating Light:
    Adjustable from 0 to 3,000 µmol (photons).m-2.s-1
  • Measuring Light:
    Red and blue measuring lights adjustable by intensity
  • Detector Wavelength Range:
    PIN photodiode with 667 to 750 nm bandpass filters
  • FluorPen 1.0 Software:
    Windows 2000, XP, or higher*
  • Memory Capacity:
    Up to 4 Mb
  • Internal Data Logging:
    Up to 100,000 data points
  • Display:
    2 x 8 characters LC display
  • Keypad:
    Sealed, 2-key tactile response
  • Keypad Escape Time:
    Turns off after 3 minutes of no use
  • Power Supply:
    4 AAA alkaline or rechargeable batteries
  • Battery Life:
    48 hours typical with full operation
  • Low Battery Detection:
    Low battery indication displayed
  • Size:
    140 x 55 x 50 mm; 5.5 " x 2.2" x 2.0"
  • Weight:
    300 g, 10.6 oz
  • Sample Holder:
    4 ml cuvette
  • Operating Conditions:
    Temperature: 0 to 55 ºC; 32 to 130 ºF. Relative humidity: 0 to 95 % (non-condensing)
  • Storage Conditions:
    Temperature: -10 to +60 ºC; 14 to +140 ºF. Relative humidity: 0 to 95 % (non-condensing)
  • Warranty:
    1 year parts and labor

* Windows is a registered trademark of Microsoft Corporation

 

AquaPen-C AP-C 100 - Price: 1.999,- €
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Mini-Computer - Price: 390,- €
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GPS Module - Price: 249,- €
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Battery Charger - Price: 49,- €
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Transport Case - Price: 79,- €
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Optional Features and Accessories:

Mini-Computer
Small, portable notebook: light-weight (1 kg), long battery runtime (about 4 hours), 8.9-inch display, 1 GB memory, integrated Bluetooth, WLAN and LAN, free Windows XP installed.

GPS Module
To record the exact location of the measurements performed by the pen-category instruments.

Battery Charger
To recharge batteries used in the pen-category instruments. Includes a battery charger and 4 AAA batteries. EU norm only.

Transport Case
Padded case to shelter all necessary accessories. Size: 28 x 24 x 8 cm.

Download Software
Download Documents
Support - your comments or questions

Other Pocket-Sized Instruments:

SpectraPen SP 100
FluorPen FP 100
PAR-FluorPen FP 100-MAX-LM
AquaPen-P AP-P 100
GFP-Pen GFP 100
PlantPen PRI 200 & NDVI 300
N-Pen N 100

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