AquaPen-P is a handheld fluorometer that enables rapid and accurate measurement of photosynthetic parameters in suspensions. It is equipped with a single blue LED emitter (optionally red or white), optically filtered and precisely focused to deliver light intensities of up to 3,000 µmol(photon).m-2.s-1 to measured algal cultures.
The measurements are carried out by a submersible optical probe. The AquaPen-P excels in a very high sensitivity - up to 0.5 µg Chl/l. Thus, it can measure even natural water samples containing low concentrations of phytoplankton. It can be favorably used both for the lab and field applications.
Data transfer is via USB or Bluetooth communication. Comprehensive FluorPen 1.0 software provides data transfer routines and many additional features for data presentation in tables and graphs. Parameters recorded by the AquaPen-P include FT, QY, NPQ, OJIP Analysis, and Light Curve.
- Photosynthesis research
- Photosynthesis education
- Phycology
- Limnology
- Oceanography
- Biotechnology
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- FT - Instantaneous Chlorophyll Fluorescence. FT is equivalent to F0 if the sample is dark-adapted.
- QY - Quantum Yield. QY is a measure of the Photosystem II efficiency. QY is equivalent to FV/FM in dark-adapted samples and to FV ' /FM ' in light-adapted samples.
- OJIP - Chlorophyll Fluorescence Transient. OJIP measurement is used as an important biophysical signal that reflects the time course of photosynthesis.
- NPQ - Non-Photochemical Quenching. NPQ indicates thermal dissipation of absorbed light energy during photosynthesis.
- LC - Light Curve. Photosystem II Quantum Yield estimated from fluorescence that is measured sequentially in several different light levels.
- Two predefined NPQ protocols differing in the duration of light exposure and dark recovery phase as well as in the number of intervals between the pulses
- Typically used for quantification of photochemical and non-photochemical quenching in dark-adapted samples
- NPQ 1 protocol: light duration 60s, 5 pulses; dark recovery duration 88s, 3 pulses
- NPQ 2 protocol: light duration 200s, 10 pulses; dark recovery duration 390s, 7 pulses
- Three predefined protocols differing in number and duration of single light phases and light intensities
- Based on pulse modulated fluorometry
- Successive measurments of the sample photosynthesis under various light intensities of continuous illumination
- Light response curve relating the rate of photosynthesis to photon flux density
- Bckg = background
- F0: = F50µs; fluorescence intensity at 50 µs
- FJ: = fluorescence intensity at j-step (at 2 ms)
- Fi: = fluorescence intensity at i-step (at 60 ms)
- FM: = maximal fluorescence intensity
- FV: = FM - F0 (maximal variable fluorescence)
- VJ = (FJ - F0) / (FM - F0)
- Vi = (Fi - F0) / (FM - F0)
- FM / F0
- FV / F0
- FV/ FM
- M0 or (dV / dt)0 = TR0 / RC - ET0 / RC = 4 (F300 - F0) / (FM - F0)
- Area = area between fluorescence curve and FM (background subtracted)
- Fix Area = total area above the OJIP fluorescence transient - between F40µ and F1s(background subtracted)
- SM = area / FM - F0 (multiple turn-over)
- Ss = the smallest Sm turn-over (single turn-over)
- N = SM . M0 . (1 / VJ) turn-over number QA
- Phi_P0 = 1 - (F0 / FM (or FV / FM)
- Psi_0 = 1 - VJ
- Phi_E0 = (1 - F0 / FM)) . Psi_0
- Phi_D0 = 1 - Phi_P0 - (F0 / FM)
- Phi_Pav = Phi_P0 - (SM / tFM); tFM) = Time to reach FM (in ms)
- ABS / RC = M0 . (1 / VJ) . (1 / Phi_P0)
- TR0 / RC = M0 . (1 / VJ)
- ET0 / RC = M0 . (1 / VJ) . Phi_0)
- DI0 / RC = (ABS / RC) - (TR0 / RC)
- Formulas Derived From:
R.J. Strasser, A. Srivastava and M. Tsimilli-Michael (2000): The fluorescence transient as a tool to characterize and screen photosynthetic samples. In: Probing Photosynthesis: Mechanism, Regulation and Adaptation (M. Yunus, U. Pathre and P. Mohanty, eds.), Taylor and Francis, UK, Chapter 25, pp 445-483. 
Transport Case
- FluorPen 1.0 software (Windows 2000, XP, or higher compatible*)
- Bluetooth, USB or serial communication
- Real-time and remote control functions
- Export to Microsoft Excel
- GPS mapping plug-in
FluorPen Software Window: OJIP Curves With Data Sheet
- Measured/Calculated Parameters:
F0 ; FT ; FM ; FM ' ; QY; OJIP; NPQ 1,2; LC 1,2,3 - Saturating Light:
Adjustable from 0 to 100 % (up to 3,000 µmol(photon).m-2.s-1 - Actinic Light:
Adjustable from 0 to 100 % (up to 1,000 µmol(photon).m-2.s-1 - Measuring Light:
Adjustable from 0 to 100 % (up to 3,000 µmol(photon).m-2.s-1 - Emitter:
Blue 455 nm LED (optionally red or white) - optically filtered and precisely focused - Detector Wavelength Range:
PIN photodiode with 667 to 750 nm bandpass filters - FluorPen 1.0 Software:
Windows 2000, XP, or higher* - Memory Capacity:
Up to 4 Mb - Internal Data Logging:
Up to 100,000 data points - Display:
2 x 8 characters LC display - Keypad:
Sealed, 2-key tactile response - Keypad Escape Time:
Turns off after 3 minutes of no use - Power Supply:
4 AAA alkaline or rechargeable batteries - Battery Life:
48 hours typical with full operation - Low Battery Detection:
Low battery indication displayed - Size:
140 x 55 x 50 mm; 5.5 " x 2.2" x 2.0" - Weight:
300 g, 10.6 oz - Sample Holder:
Submersible optical probe - 80 mm long; lightguide diameter 8 mm; lightguide end 15 mm in diameter - Operating Conditions:
Temperature: 0 to 55 ºC; 32 to 130 ºF. Relative humidity: 0 to 95 % (non-condensing) - Storage Conditions:
Temperature: -10 to +60 ºC; 14 to +140 ºF. Relative humidity: 0 to 95 % (non-condensing) - Warranty:
1 year parts and labor
Pricing
- Orders and Payments
- AquaPen-P AP-P 100 1.999,- €
- Mini-Computer * 390,- €
- GPS Module * 249,- €
- Battery Charger * 49,- €
- Transport Case * 79,- €
Optional Features and Accessories
Mini-Computer
Small, portable notebook: light-weight (1 kg), long battery runtime (about 4 hours), 10.1-inch display, 1 GB memory, integrated Bluetooth, WLAN and LAN, Windows 7 installed.
GPS Module
To record the exact location of the measurements performed by the pen-category instruments.
Battery Charger
To recharge batteries used in the pen-category instruments. Includes a battery charger and 4 AAA batteries. EU norm only.
Transport Case
Padded case to shelter all necessary accessories. Size: 25 x 22 x 5 cm.
Download
FluorPen 1.0
OS: Win2000/XP
Language: English
Size: 2.2MB
Documents
FluorPen Series Manual
Type: PDF
Language: English
Size: 1.2MB
FluorPen Quick Guide
Type: PDF
Language: English
Size: 82kB
FluorPen Explain
Type: PDF
Language: English
Size: 2.9MB
SpectraPen Manual
Type: PDF
Language: English
Size: 247kB
AquaPen Manual
Type: PDF
Language: English
Size: 1.3MB
AquaPen Quick Guide
Type: PDF
Language: English
Size: 85kB
N-Pen Brief Guide
Type: PDF
Language: English
Size: 106kB
GFP-Pen Manual
Type: PDF
Language: English
Size: 812kB
PlantPen NDVI Manual
Type: PDF
Language: English
Size: 363kB
PlantPen PRI Manual
Type: PDF
Language: English
Size: 390kB
PlantPen NDVI, PRI Quick Guide
Type: PDF
Language: English
Size: 73,7kB
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