AquaPen-P AP-P 100

AquaPenAquaPen-P is a handheld fluorometer that enables rapid and accurate measurement of photosynthetic parameters in suspensions. It is equipped with a blue measuring light that suits investigations in algal suspensions.

The measurements are carried out by a submersible optical probe. The AquaPen-P excels in a very high sensitivity - up to 10 ng Chl/l. Thus, it can measure even natural water samples containing low concentrations of phytoplankton. It can be favorably used both for the lab and field applications.

Incorporated Bluetooth/USB/serial communication technology and the FluorPen 1.0 software provide visualization and data transfer routines to a PC.

Applications:

  • Photosynthesis research
  • Photosynthesis education
  • Phycology
  • Limnology
  • Oceanography
  • Biotechnology

AquaPen-P Measures:

  • FT - Instantaneous Chlorophyll Fluorescence. FT is equivalent to F0 if the sample is dark-adapted.
  • QY - Quantum Yield. QY is a measure of the Photosystem II efficiency. QY is equivalent to FV/FM in dark-adapted samples and to FV ' /FM ' in light-adapted samples.
  • OJIP - Chlorophyll Fluorescence Transient. OJIP measurement is used as an important biophysical signal that reflects the time course of photosynthesis.
  • NPQ - Non-Photochemical Quenching. NPQ indicates thermal dissipation of absorbed light energy during photosynthesis.
  • LC - Light Curve. Photosystem II Quantum Yield estimated from fluorescence that is measured sequentially in several different light levels.

NPQ Protocol
NPQ Protocol Includes Five Measurements in Actinic Light
and Three Measurements During Dark Relaxation.
NPQ_Ln = (FM - FM_Ln) / FM_Ln
NPQ_Lss = (FM - FM_Lss) / FM_Lss
NPQ_Dn = (FM – FM_Dn) / FM_Dn

Light Curve Protocol
Adaptation of QY to Six Different Light Levels

Software:

  • FluorPen 1.0 software (Windows 2000, XP, or higher compatible*)
  • Bluetooth, USB or serial communication
  • Visualization and data transfer routines to Microsoft Excel
  • Real-time and remote control functions
  • GPS mapping plug-in

Calculated parameters
FluorPen Software Window: OJIP Curves With Data Sheet

Explanation of OJIP Parameters:

  • Bckg = background
  • F0: = F50µs; fluorescence intensity at 50 µs
  • FJ: = fluorescence intensity at j-step (at 2 ms)
  • Fi: = fluorescence intensity at i-step (at 60 ms)
  • FM: = maximal fluorescence intensity
  • FV: = FM - F0 (maximal variable fluorescence)
  • VJ = (FJ - F0) / (FM - F0)
  • Vi = (Fi - F0) / (FM - F0)
  • FM / F0
  • FV / F0
  • FV/ FM
  • M0 or (dV / dt)0 = TR0 / RC - ET0 / RC = 4 (F300 - F0) / (FM - F0)
  • Area = area between fluorescence curve and FM (background subtracted)
  • Fix Area = total area above the OJIP fluorescence transient - between F40µ and F1s(background subtracted)
  • SM = area / FM - F0 (multiple turn-over)
  • Ss = the smallest Sm turn-over (single turn-over)
  • N = SM . M0 . (1 / VJ) turn-over number QA
  • Phi_P0 = 1 - (F0 / FM (or FV / FM)
  • Psi_0 = 1 - VJ
  • Phi_E0 = (1 - F0 / FM)) . Psi_0
  • Phi_D0 = 1 - Phi_P0 - (F0 / FM)
  • Phi_Pav = Phi_P0 - (SM / tFM); tFM) = Time to reach FM (in ms)
  • ABS / RC = M0 . (1 / VJ) . (1 / Phi_P0)
  • TR0 / RC = M0 . (1 / VJ)
  • ET0 / RC = M0 . (1 / VJ) . Phi_0)
  • DI0 / RC = (ABS / RC) - (TR0 / RC)

Formulas Derived From:
R.J. Strasser, A. Srivastava and M. Tsimilli-Michael (2000): The fluorescence transient as a tool to characterize and screen photosynthetic samples. In: Probing Photosynthesis: Mechanism, Regulation and Adaptation (M. Yunus, U. Pathre and P. Mohanty, eds.), Taylor and Francis, UK, Chapter 25, pp 445-483.

 


Transport Case

Technical Specification:

  • Measured/Calculated Parameters:
    F0 , FT , FM , FM ' , QY, OJIP, NPQ, LC 1, LC 2, OD 680 , OD 720
  • Actinic and Saturating Light:
    Adjustable from 0 to 3,000 µmol (photons).m-2.s-1
  • Measuring Light:
    Blue measuring light adjustable by intensity
  • Detector Wavelength Range:
    PIN photodiode with 667 to 750 nm bandpass filters
  • FluorPen 1.0 Software:
    Windows 2000, XP, or higher*
  • Memory Capacity:
    Up to 4 Mb
  • Internal Data Logging:
    Up to 100,000 data points
  • Display:
    2 x 8 characters LC display
  • Keypad:
    Sealed, 2-key tactile response
  • Keypad Escape Time:
    Turns off after 3 minutes of no use
  • Power Supply:
    4 AAA alkaline or rechargeable batteries
  • Battery Life:
    48 hours typical with full operation
  • Low Battery Detection:
    Low battery indication displayed
  • Size:
    140 x 55 x 50 mm; 5.5 " x 2.2" x 2.0"
  • Weight:
    300 g, 10.6 oz
  • Sample Holder:
    Submersible optical probe
  • Operating Conditions:
    Temperature: 0 to 55 ºC; 32 to 130 ºF. Relative humidity: 0 to 95 % (non-condensing)
  • Storage Conditions:
    Temperature: -10 to +60 ºC; 14 to +140 ºF. Relative humidity: 0 to 95 % (non-condensing)
  • Warranty:
    1 year parts and labor

* Windows is a registered trademark of Microsoft Corporation

 

AquaPen-P AP-P 100 - Price: 1.999,- €
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Mini-Computer - Price: 390,- €
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GPS Module - Price: 249,- €
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Battery Charger - Price: 49,- €
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Transport Case - Price: 79,- €
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Optional Features and Accessories:

Mini-Computer
Small, portable notebook: light-weight (1 kg), long battery runtime (about 4 hours), 8.9-inch display, 1 GB memory, integrated Bluetooth, WLAN and LAN, free Windows XP installed.

GPS Module
To record the exact location of the measurements performed by the pen-category instruments.

Battery Charger
To recharge batteries used in the pen-category instruments. Includes a battery charger and 4 AAA batteries. EU norm only.

Transport Case
Padded case to shelter all necessary accessories. Size: 25 x 22 x 5 cm

Download Software
Download Documents
Support - your comments or questions

Other Pocket-Sized Instruments:

SpectraPen SP 100
FluorPen FP 100
PAR-FluorPen FP 100-MAX-LM
AquaPen-C AP-C 100
GFP-Pen GFP 100
PlantPen PRI 200 & NDVI 300
N-Pen N 100

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