- Cultivation vessel capacity:
400 ml (in FMT 150/400)
1000 ml (in FMT 150/1000). - Programmable light, temperature, gas, and medium regime. Time steps from milliseconds to hours.
- Both static and changeable temperature in the range of 18 - 50 °C (standard) or in the range of 0 - 70 °C (with optional accessories).
- Controlled flow and composition of the bubbling air (with optional accessories).
- Magnetic stirring (with optional accessories).
- Bubble interruption during measurements - bubble interruption valve (with optional accessories).
- Bubble humidifier to keep the volume in the vessel stable (with optional accessories).
- Up to 8 peristaltic pumps for different chemostat or pH-stat cultivation control (with optional accessories).
- Continuous-flow turbidostatic cultivation - stabilization of the suspensions, optical density control (with optional accessories).
- Full software control enabling creation of user-defined protocols (with optional accessories).
Photobioreactor with Accessories
- Standard panel is included in the Photobioreactor unit price.
A customer can choose from two panel versions, in which each color is separately controllable:
- White-Red panel: maximum intensity 1,500 µmol(photon)/m2.s (white 750 / red 750)
- Blue-Red panel: maximum intensity 1,500 µmol(photon)/m2.s (blue 750 / red 750) - Light Upgraded panel is an option and must be ordered separately.
A customer can choose from two basic versions, in which each color is separately controllable:
- White-Red panel: maximum intensity 3,000 µmol(photon)/m2.s (white 2250 / red 750)
- Blue-Red panel: maximum intensity 3,000 µmol(photon)/m2.s (blue 2250 / red 750) - Besides that, the following customized panels are offered (please inquire for their price).
- White-Blue: maximum intensity 1,500 µmol(photon)/m2.s
- White-Blue with Light Upgrade: maximum intensity 3,000 µmol(photon)/m2.s
- Red: maximum intensity 750 µmol(photon)/m2.s (no light upgrade available)
- White: maximum intensity 1,500 µmol(photon)/m2.s
- White with Light Upgrade: maximum intensity 3,000 µmol(photon)/m2.s
- Blue: maximum intensity 1,500 µmol(photon)/m2.s
- Blue with Light Upgrade: maximum intensity 3,000 µmol(photon)/m2.s - As an option, External Light Panel may be mounted to the Photobioreactor front part. This panel may be used to accommodate specific needs of different organisms or to increase light intensity (optional; various wavelengths available; offered in sigle-color or bi-color versions).
- Illumination is software controlled and enables both static and fluctuating light regime.
- Timing enables day / night cycles.
- Integrated Double-Modulation Fluorometer with online measurement and recording of basic fluorescence parameters - Ft, Fm, QY (standard).
- Continuous temperature control (standard).
- Integrated online optical density measurement (standard).
- O2 electrode to measure concentration of dissolved O2 (with optional accessories).
- CO2 electrode to determine dissolved CO2 concentration (with optional accessories).
- Continuous pH monitoring (with optional accessories).
- Gas Analyzing System for CO2 and O2 - gas output measurement (with optional accessories).
- High-precision Gas Mixing System (with optional accessories).
Gas Mixing System GMS 150
- User-friendly operation.
- Robust construction, thermal stability, long-life light source.
- Computer control or manual control.
- Remote control via internet.
- Many useful accessories.
- User-defined protocol writing.
- Data collection in real time.
- Data upload even when the experiment is running.
- Data visualization in graphs.
- Further processing of data sheets in Excel accessible format.
- Remote control of the experiment via internet.
RCM Software
- Online Measured Parameters:
Fluorescence - Ft, Fm, QY (standard)
temperature (standard)
optical density (standard)
pH, O2, CO2 (optional) - Vessel Capacity:
400 ml / 1000 ml - Precision Controlled Temperature:
18 °C - 50 °C (standard)
0 °C - 70 °C (optional) - Cooling and Heating System:
Two 200 W Peltier elements - Two Displays:
System control
Actual readings - LED Lighting:
Light intensity adjustable from 0 to 100 %
Bi-color light panels available (separate color control) - Light Regime:
Static or dynamic (sinus form) - Optical Density Measurement:
Real time measurement of OD by two IR LEDs (735 nm, 680 nm) - Detector Wavelength Range:
PIN photodiode with 697 nm-750 nm bandpass filters - Controlled Flow and Composition of Bubbled Air:
Optional - Pumps:
Up to 8 peristaltic pumps (optional) - Optional Probes:
ph/temperature, O2 , CO2 - Bios:
Upgradeable firmware - Communication Port:
Serial - RS232 - Material:
Glass, stainless steel, silicon gasket - Dimension:
50 x 25 x 35 cm - Weight:
15.5 kg - Electrical:
90-240 V
- Nedbal L. et al. (2008): Biotechnology and Bioengineering 100: 902-910
- Cerveny J. et al. (2009): Eng. Life Sci., No. 3, 247-253
- Cerveny J., Nedbal L. (2009): J. Biol. Rhythms, Vol. 24, No. 4, 295-303
- Nedbal L. et al.(2010): J. Ind. Microbiol. Biotechnol., 37: 1319-1326
Pricing
- Orders and Payments
- Photobioreactor FMT 150/400 11.999,- €
- Photobioreactor FMT 150/1000 14.999,- €
- Light Upgrade 1.990,- €
- Turbidostat Module I. 1.990,- €
- Turbidostat Module II. 2.390,- €
- Peristaltic Pump PP 500 1.190,- €
- Peristaltic Pump PP 550 1.490,- €
- PWM Pump 680,- €
- High-Precision Gas Mixing System 8.680,- €
- Gas Analyzing System 11.890,- €
- O2 Module * 1.980,- €
- CO2 Module * 2.980,- €
- pH/Temperature Module * 1.290,- €
- Additional Cable to Probes 250,- €
- Magnetic Stirrer 990,- €
- Air Pump with Bubble Interruption Valve 230,- €
- Bubble Humidifier 59,- €
- External Light Panel E-1 1.990,- €
- External Light Panel E-2 2.390,- €
- External Light Panel E-3 2.990,- €
- Supplementary Vessel (standard model, 400 ml) 470,- €
- Supplementary Vessel (standard model, 1000 ml) 850,- €
- Supplementary Glass Part to Cultivation Vessel (400 ml) 190,- €
- Supplementary Glass Part to Cultivation Vessel (1000 ml) 290,- €
- Enhanced Vessel (400 ml) 890,- €
- Enhanced Vessel (1000 ml) 1.250,- €
- Supplementary Lid to Cultivation Vessel 200,- €
- Spare Part Kit * 65,- €
- Shelf Unit 1.090,- €
- Photobioreactor Control Unit 2.900,- €
- Additional Software License 300,- €
Optional Features and Accessories
Light Upgrade
To increase intensity of actinic light in the Photobioreactor. The maximum reachable depends on light color - please inquire at zc.isp@ofni.
Turbidostat Module I.
Includes Peristaltic Pump PP 500 and controller. The module enables continuous-flow turbidostatic cultivation. In the most basic setup, the Photobioreactor optical sensing unit monitors the optical density (OD680, OD735) of the culture. The Peristaltic Pump adds medium accordingly to keep the culture turbidity constant.
Turbidostat Module II.
Includes Peristaltic Pump PP 550 and controller. The module enables continuous-flow turbidostatic cultivation with additional possibility of gas regulation, for instance, for pH-stat management via changing gas rate.
Peristaltic Pump PP 500
The system enables cascading of up to 8 pumps. These pumps can be operated in pH-stat or chemostat mode and each pump can be controlled according to a different parameter (pH, Ft, Fm...). The pump allows variable flow-rate range from 10 to 5600 µl/min.
Peristaltic Pump PP 550
The enhanced pump version can control both liquids and gases. It may be equipped with up to four electrical valves that may serve for regulation of gas inflow into the culture (pH-stat or chemostat cultivation). PP 550 is designed to be used with the parent device only. The standard version comes with one electrical valve; up to three additional valves may be enabled as an option.
PWM Pump (for Gas or Liquid)
PWM pump can be used for pumping liquid or gas into the cultivation cuvette; it can be regulated withing a range of 1-100 % of total output. The pump has diverse uses: (1) Controlled air bubbling in the cultivation vessel; (2) Single gas input into the vessel; (3) When connected to the vessel inlet and outlet, it can be used for suspension mixing (bubbling) in anaerobic conditions.
O2 Module
To measure concentration of dissolved O2 (includes the probe and a special electronic module).
CO2 Module
To determine the dissolved carbon dioxide concentration in the medium (includes the probe and a special electronic module). This gas sensing electrode consists of a pH electrode immersed in an electrolyte solution which is separated from the measuring solution by a gas sensitive membrane.
pH / Temperature Module
To measure pH and temperature (includes the probe and a special electronic module).
Additional Cable to Probes
Supplementary cable and connector for O2 probe.
Air Pump with Bubble Interruption Valve
Air pump for culture bubble stirring. Supplemented with a valve, which interrupts bubbling during single measurements, and thus allows for more precise readings.
Magnetic Stirrer
The magnetic stirrer rotates a teflon-coated magnetic bar in a vertical plane of the front window inside the Photobioreactor. The frequency of the rotation is adjustable. The stirrer is essential when the agitation by air or gas bubbles cannot be used, e.g., when the photosynthetic oxygen evolution or respiratory oxygen uptake are measured. It can be also used to reduce cell sedimentation in addition to agitation by air or other gas bubbles.
Bubble Humidifier
To keep the vessel volume stable.
External Light Panel
To accommodate specific needs of different organisms or to increase illumination intensity. External light panel is offered in three versions: single color (E-1), single color with additional IR LEDs (E-2), and bicolor (E-3).
Standard Supplementary Vessel
Suitable for cultivation in the range of 18 - 50 °C. Made from stainless steel and glass, it includes the frame, lid and glass body. Supplementary glass part includes just the glass body.
Enhanced Vessel
Suitable for cultivation in the range of 0 - 70 °C. Made from nickel-plated brass covered with ceramic layer.
High-Precision Gas Mixing System
High-precision gas mixer can hold up to four channels. The standard version of the system includes two channels: Channel 1 serves Air-N2; Channel 2 serves CO2.
Gas Analyzing System GAS 150
Enables measuring CO2 and O2 at the cultivation output. If connected to CO2 electrode, it also enables measuring CO2 in the cultivated suspension.
Spare Part Kit
Kit includes: bulk connection plastics 45pc, one-way valves 5pc, double-check valve 5pc, stainless bushing with silicone ring 2pc, rubber gasket 5pc, o-rings 5pc, silicone tubing 10 m.
Shelf Unit
Heavy duty shelf unit can accommodate and organize the Photobioreactor with all necessary accessories.
Photobioreactor Control Unit
Includes a PC with software for data collection and data visualization. The software enables: Writing of user-defined protocols; Data collection in real time; Data upload for processing even when the experiment is running (in Excel accessible format; Data visualization in graphs; Remote control via internet.
Addional Software License
A separate license required for each additional Photobioreactor software control.
Download
Nothing
Documents
Photobioreactor Manual
Type: PDF
Language: English
Size: 22MB
Multi-Cultivator Manual
Type: PDF
Language: English
Size: 2.4MB
Other Photobioreactors
Photobioreactors FMT 150/400 and FMT 150/1000 feature a unique combination of the cultivator and computer controlled monitoring device. FMT 150/400 and FMT 150/1000 differ in the capacity of their cultivation vessels - 400 ml and 1000 ml respectively. Both Photobioreactor models may be supplied with a number of useful accessories - sensors, stirrers, pumps, etc.
Multi-Cultivator MC 1000
Multi-Cultivator MC 1000 serves for small scale cultivation of algae, bacteria or cyanobacteria. The instrument represents a perfect solution in applications requiring diverse experimental variants. In an ideal configuration, the high-throughput part of the experiments is performed in the MC 1000 and the results are finalized in our FMT 150.
Large-Scale Photobioreactors
Large-volume bioreactors are manufactured with respect to customer's specific needs and they come with a number of useful accessories. Two basic versions feature:
- 25-liter cultivation tank
- 120-liter cultivation tank