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Photobioreactor FMT 150 With Integrated Fluorometer and Densitometer

Key Features - Cultivation:

• Cultivation vessel capacity:
  400 ml (in FMT 150)
  1000 ml (in FMT 150/1000).
• Programmable light, temperature, gas, and medium regime. Time steps from milliseconds to hours.
• Both static and changeable temperature in the range of 18 - 50 °C (standard) or in the range of 0 - 70 °C (optional).
• Controlled flow and composition of the bubbling air (optional).
• Magnetic stirring (optional).
• Bubble interruption during measurements - bubble interruption valve (optional).
• Bubble humidifier to keep the volume in the vessel stable (optional).
• Peristaltic pumps for chemostate-cultivation, i.e., steady-state growing of cultures (optional).
• Turbidostat cultivation - stabilization of the suspensions, optical density control (optional).

Photobioreactor With Accessories

Key Features - LED Lighting:

• Standard color versions :
  - White
  - Red-blue (630 nm, 450 nm)
  - White-red (630 nm).
• Other colors/color combinations (optional).
• Both static and fluctuating light regime.
• Actinic light up to 600 µmol(photon)/m².s (standard).
• Light intensity upgrade from 1,800 - 2,500 µmol(photon)/m².s; according to LED type and color (optional).
• Wide range of wavelengths from UV-A through VIS to NIR (optional).
• Additional light source to accommodate specific needs of different organisms. For instance, Infra Red (850 nm) light source for purple sulfur photosynthetic bacteria. (optional; various wavelengths available).

Key Features - Monitoring:

• Integrated Double-Modulation Fluorometer with online measurement and recording of fluorescence parameters, such as Fo, Ft, Fm, Fm´, (Fm’-Ft)/Fm’, and others (standard).
• Integrated online optical density measurement (standard).
• O₂ / H₂ electrode to measure concentration of dissolved gases (optional).
• CO₂ electrode to determine the dissolved CO₂ concentration in the Photobioreactor medium (optional).
• Online pH monitoring (optional).
• Magnetic Stirrer (optional).
• High-precision Gas Mixing System (optional).

Gas Mixing System GMS 150

Other Features:

• User-friendly operation.
• Robust construction, thermal stability, long-life light source.
• PC interface.
• Remote control.

Block Scheme of the Device and Its External Modules

Control Software:

• Data collection in real time.
• Data upload even when the experiment is running.
• Data visualization in graphs.
• Further processing of data sheets in Excel accessible format.
• Remote control of the experiment via internet.

RCM Software

Shelf Unit

Technical Specification:

• Fluorescence Parameters:
  Fo, Ft, Fm, Fm´, Fv/Fm. Other parameters can be calculated.
• Vessel Capacity / Weight:
  400 ml / 3 kg.
• Precision Controlled Temperature:
  5 °C - 60 °C.
• Temperature Sensor:
  Platinum Pt 1,000 in thermo-well.
• Cooling and Heating System:
  Two 200 W Peltier elements.
• Two Displays:
  For system control and actual temperature.
• Actinic and Saturating Light:
  Two standard versions: one with blue and red LEDs (450 nm, 630 nm); the other with white LEDs. Light intensity is adjustable from 0 to 2,500 µmol(photon)/m².s. Other LED colors available as an option.
• Light Regime:
  Static or dynamic (sinus form).
• OD - Optical Density Measurement:
  Real time measurement of OD by two IR LEDs (735 nm, 680 nm).
• Detector Wavelength Range:
  PIN photodiode with 697 nm-750 nm bandpass filters.
• Controlled Flow and Composition of the Bubbling Air:
  Optional.
• Pumps:
  Up to 7 peristaltic pumps (optional).
• Ph Probe:
  Optional.
• Bios:
  Upgradeable firmware.
• Communication Port:
  Serial - RS232.
• Material:
  Glass, stainless steel, silikon gasket.
• Size:
  50 x 25 x 35 cm.
• Weight:
  15.5 kg.
• Electrical:
  90-240 V.

References:

• Nedbal L. et al. (2008): Biotechnology and Bioengineering 100: 902-910.
• Cerveny J. et al. (2009): Eng. Life Sci., No. 3, 247-253.
• Cerveny J., Nedbal L. (2009): J. Biol. Rhythms, Vol. 24, No. 4, 295-303.