High-Sensitivity Fluorometer FL 3500/HS
The High-Sensitivity Fluorometer with its detection limit of 1 ng Chl/l allows measuring of fluorescence induction and of QA reoxidation kinetics with pico-molar chlorophyll concentrations (Koblizek et al. 2005, FEMS Microbiology Ecology 51, 353-361). The typical experimental material is natural water with phytoplankton.
The core of the instrument is an integrating Teflon sphere containing the liquid sample. The optical ports of the sphere are supplied with three sets of light-emitting diodes and with an avalanche photodiode detector. As an option, the instrument can be supplied with a second detector to capture fluorescence transients in alternative spectral band. Contact PSI for your specific needs. Measuring flashes, single-turnover saturating flashes, and continuous actinic light are of blue color, λmax=455 nm. Light intensities and timing are software controlled separately for each LED set.
Typical Samples:
- Natural water with phytoplankton
- Chloroplasts & thylakoids
- Algae & cyanobacteria
Fluorometer-Supported Investigations:
- Fluorescence induction
- Pulse amplitude modulation measurements (PAM)
- Fast OJIP transient capture
- Rapid measurements of QA-reoxidation kinetics
- State transitions
- Quenching parameters
- Photochemical yields
- Determination of the effective antenna size in Photosystem II
State Transitions Capture
FluorWin Software:
- Creation and archivation of experimental protocols.
- FluorWin Wizard for automated protocols.
- Retrieval and export of experimental data.
- Data manipulation and visualization.
FluorWin Software: Wizard / Graph Window
References:
- Koblizek M. et al. (2005): FEMS Microbiology Ecology 51 (3): 353–361.
