High-Sensitivity Fluorometer with its detection limit of 1 ng Chl/l allows measuring of fluorescence induction and of QA reoxidation kinetics with pico-molar chlorophyll concentrations (Koblizek et al. 2005, FEMS Microbiology Ecology 51, 353-361). The typical experimental material is natural water with phytoplankton.
The core of the instrument is an integrating Teflon sphere containing the liquid sample. The optical ports of the sphere are supplied with three sets of light-emitting diodes and with an avalanche photodiode detector. As an option, the instrument can be supplied with a second detector to capture fluorescence transients in alternative spectral band. Contact PSI for your specific needs. Measuring flashes, single-turnover saturating flashes, and continuous actinic light are of blue color, λmax=455 nm. Light intensities and timing are software controlled separately for each LED set.

- Natural water with phytoplankton
- Chloroplasts & thylakoids
- Algae & cyanobacteria
- Fluorescence induction
- Pulse amplitude modulation measurements (PAM)
- Fast OJIP transient capture
- Rapid measurements of QA-reoxidation kinetics
- State transitions
- Quenching parameters
- Photochemical yields
- Determination of the effective antenna size in Photosystem II

State Transitions Capture
- Creation and archivation of experimental protocols.
- FluorWin Wizard for automated protocols.
- Retrieval and export of experimental data.
- Data manipulation and visualization.


FluorWin Software: Wizard / Graph Window
- Koblizek M. et al. (2005): FEMS Microbiology Ecology 51 (3): 353–361.
Pricing
- Orders and Payments
- High-Sensitivity Fluorometer FL3500/HS 14.990,- €
Download
FluorWin 3.6
OS: Win2000/XP
Language: English
Size: 2.2MB
Documents
Fluorometer FL3000X User's Guide
Type: PDF
Language: English
Size: 598kB
Algal Online Monitor Operation Manual
Type: PDF
Language: English
Size: 2MB
Other Fluorometers
Highly compact:
Measuring optical head with a standard cuvette for sample suspensions of aquatic or terrestrial plants.
Typical samples:
Suspensions of photosynthetically active organisms, small leaves, or leaf segments.
SuperHead Fast FL 3500/F
Supported investigations:
- PAM measurements
- Fast kinetic measurements of OJIP
- Flash fluorescence induction
- State transitions etc.
Time resolution of 1 µs.
Algal Online Monitor
Flow-through monitoring:
Enables detection and continuous quantifying of photosynthetically active microorganisms in rivers, lakes, or artificial water reservoirs.
Detection limit: 30 ng Chl/l.
Submersible FL 3500/SM
Underwater experiments:
Maximum operating depth of the instrument is ca 1 meter. The measuring compartment is equipped with a practical holder.
Typical samples:
Plants, corals and seaweed.
Leaf-Clip FL 3500/LC
Measuring in leaves:
Equipped with a non-intrusive leaf-clip, this version is designed to measure fluorescence of plant leaves or their segments.
Measured area:
circle 12.7 mm (0.5 inch) in diameter.
High Sensitivity FL 3500/HS
Highlights:
- High sensitivity
- Detection limit: 1 ng Chl/l
- Fluorescence induction measurements
- QA-reoxidation kinetics in pico-molar chlorophyll concentrations.
Online Ultra-High Sensitivity FL 5000/US-O
Highlights:
- Compact, powerful system
- Simple operation via the touch screen
- Utra-high sensitivity
- Detection limit: 0.1 ng Chl/l
- Web interface.