Double-Modulation Fluorometer FL 3500 measures Chl-fluorescence signal with a time resolution of up to 1 µs in the Fast FL3500/F version or up to 4 µs in the Standard FL3500/S version. Measured fluorescence emission is excited by a set of light-emitting diodes that generate short measuring flashes; the photochemistry is driven by single-turnover flashes or by continuous actinic irradiance. Chlorophyll fluorescence is detected by a PIN photodiode and digitized by a 16-bit A/D converter. The instrument supports Pulse Amplitude Modulation measurements and, at the same time, can capture fast OJIP transients or perform rapid measurements of QA- reoxidation kinetics, S-states, or of the effective antenna size. The major novel feature of this fluorometer is the capacity of the instrument to generate rectangular actinic flashes of extremely high power. Full reduction of QA acceptor can be achieved within 25 µs and the instrument can measure fluorescence induction during such a single-turnover saturating flash. This technique is used to determine the effective antenna size of the Photosystem II as well as its heterogeneity and connectivity without disturbing the measured system by DCMU or other herbicides. Photosynthetically active samples, either suspensions, leaves, or even corals, can be investigated for their photochemical yields, quenching parameters, state transitions, or for the kinetics of photosynthetic redox reactions.
PSI fluorometers measure:
Various Chl fluorescence parameters, ratios, and quenching coefficients that provide information on the functionality of the photosynthetic apparatus. These are five basic fluorescence parameters - F0, Fm, F0, Fm', Fs - and a number of differences and fluorescence ratios. Such as, for instance:
- Maximum quantum yield of Photosystem II
- Non-photochemical quenching NPQ
- Effective quantum yield of Photosystem II
- Non-photochemical quenching of variable Chl fluorescence
- Photochemical quenching of variable Chl fluorescence
Other supported protocols:
- Flash fluorescence induction (one flash)
- Fast OJIP transients
- Rapid QA-reoxidation kinetics
- Effective antenna size
- PSII connectivity (J parameter)
How PSI fluorometers measure:
Measured fluorescence emission is excited by a set of light emitting diodes that generate measuring flashes. The photochemistry is driven by single-turnover flashes or by continuous actinic irradiance. The FL3500 has two input channels. The first channel is used for Chla-fluorescence measurements. The second channel can be optionally used for temperature reading or dissolved oxygen probe signal measurements. FAR-LED unit with 735 nm LEDs can be optionally connected to the SuperHead measuring unit. Thermoregulator TR 2000 or dissolved O2 microelectrode A/D converter are optional accessories for measurements with the Fluorometer measuring unit.
Measuring optical head with a standard cuvette for sample suspensions of aquatic or terrestrial plants.
Suspensions of photosynthetically active organisms, small leaves, or leaf segments.
- PAM measurements
- Fast kinetic measurements of OJIP
- Flash fluorescence induction
- State transitions etc.
Time resolution of 1 µs.
Enables detection and continuous quantifying of photosynthetically active microorganisms in rivers, lakes, or artificial water reservoirs.
Detection limit: 30 ng Chl/l.
Standard Fluorometer FL 3500-S
|Includes measuring optical head with a cuvette, multipurpose control unit, power supply, FluorWin software, user’s guide.||8.490 €|
Fast Fluorometer FL 3500-F
|Includes measuring optical head with a cuvette, multipurpose control unit, power supply, FluorWin software, user’s guide.||9.990 €|
Algal Online Monitor
|Robust, portable device for flow-through measuring of fluorescence. Includes FluorPen 1.0 software for data collection and external communication.||7.990 €|
Fluorometer Optional Accessories - Infra-Red LED Unit
|Allows measuring Fo' and PAR absorbance. It is also used during quenching analysis protocol.||1.490 €|
Fluorometer Optional Accessories - SuperHead
|Fluorometer standard configuration can be enhanced by adding a second SuperHead. This SuperHead can be constructed with respect to customer's experimental needs: specific LED colors (Blue, Cyan, Amber) and detection bands (ChlA, ChlB).||3.490 €|
Fluorometer Optional Accessories - Thermoregulator TR 2000
|Includes control unit and temperature controller.||2.999 €|
Fluorometer Optional Accessories - Magnetic Stirrer
|For uniform sample stirring. Can be controlled manually or by the Fluorometer Control Unit.||998 €|
Fluorometer Optional Accessories - Oxygen Detector Module
|Serves for oxygen evolution detection based on dynamic quenching of fluorescence (no electrode included).||1.550 €|
Fluorometer Optional Accessories - Oxygen Electrode *
|A supplement to oxygen detector module.||690 €|