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Open FluorCam FC 800-O/1010

Open FluorCam is a highly modular instrument with flexible geometry. The LED panels and the light source generating saturating flashes can be arranged at various angles and distances from the sample. The position of the camera may also be adjusted for added precision. The maximum area for imaging with standard hardware is 13 x 13 cm, respectively 20 x 20 cm, depending on the size of selected light sources. Much larger areas, up to 200 x 100 cm, can be imaged by the addition of LED panels and/or by mounting the whole system on a movable frame - see the XY-Plane, Transect, Arch, and Rover Customized FluorCams, or Open FluorCam video on this page.

Applications:

• Screening for photosynthetic performance.
• Stress resistance or susceptibility.
• Stomatal patchiness.
• Metabolic perturbations.
• Growth and yield.

Samples:

• Leaves, plants, fruits, vegetables.
• Cyanobacteria, green algae.
• Sample size up to 13 cm x 13 cm, respectively 20 x 20 cm.
• Imaging masks for 384-well plate, 96-well plate, Petri dish, etc.
• Dark room for adaptation as an option.

Wheat Leaf Image

Experiments and Measured Parameters:

• Quenching.
• Kautsky induction.
• QA reoxidation (needs optional accessories).
• OJIP (needs optional accessories).
• Fast fluorescence induction with 1µs resolution (needs optional accessories).
• PAR absorptivity (needs optional accessories).
• Measured parameters: Fo, FM, FV, Fo’, FM’, FV’, QY(II).
• More than 50 calculated parameters: NPQ, FV/FM, FV’/FM’, Rfd, qN, qP, PAR-absorptivity, electron transport rate (ETR), and many others.

Different Emission Bands:

• 8 position filter wheel (optional).
• Chlorophyll fluorescence (high pass 695 nm, low pass 780 nm), GFP (high pass 495, low pass 660 nm, band pass 505/560 nm), PAR (clear glass), YFP, CY3, CY5, as well as other fluorescence colors.

Images of More Than 50 Calculated Parameters in One Measurement

Light Sources:

• Actinic light up to 3,000 µmol(photons)/m².s.
• Super pulse up to 7,500 µmol(photons)/m².s.
• STF - Single Turnover Flash.
• IR 735 nm (FAR).
• The FluorCam system includes four super bright LED panels.
• Two panels provide Measuring Light and Actinic Light 1 (red light 618 nm in a standard version). Two panels provide Actinic Light 2 and Saturating Pulse ( these two panels are mounted according to customer's needs in blue (455 nm), red (618 nm), or white color).
• Other wavelengths are available as option: 390 nm, 470 nm, 505 nm, 570 nm, 605 nm, 630 nm, 735 nm, and others.
• Additional light sources, mounted close to the camera stand or to the device side, may be provided, e.g., infra-red, ultra-violet, or green (optional).
• Variable excitation color.

Standard Imaging Format:

• 512 x 512 pixels.
• A/D: 12 bit (4096 grey levels).
• 8.2 µm x 8.4 µm pixel size.
• 50 images per second.
• Advantageous for rapid processes measurements.

Optional Imaging Format:

• 640 x 480 pixels and 1392 x 1040 pixels, respectively.
• A/D: 12 bit (4096 grey levels).
• 6.45 µm x 6.45 µm pixel size.
• 30 and 15 images per second, respectively.
• Great for slow processes measurements and for applications where the higher spatial resolution is of high importance.
• Download more information here.

FluorCam Software Window

NEW FluorCam 7.0 Software

• Automated experimental protocols via a Windows Wizard.
• Multiple (automatically repeated) experiments.
• Automated labeling of individual plants, or samples, within the field of view.
• Kinetic analysis of data from all samples within the field of view.
• Numerous image manipulation tools.
• Barcode reader support.
• Export to Excel.
• Windows 2000, XP, Vista compatible*.

* Windows 2000, Windows XP and Vista are trademarks of Microsoft Corporation.

Dark Adaptation Box

Technical Specification:

• Fluorescence parameters
  (F0, FM, FV, FO', FM', FV', QY(II)), Abs PAR-value, or the parameters that are calculated from fluorescence emission (e.g., NPQ, FV/FM, FV'/FM', Rfd, qN, qP), PAR-absorptivity, photosynthetic electron transport rate (PS), and others
• Excitation light sources
  390 nm, 450 nm, 470 nm, 505 nm, 570 nm, 605 nm, 630 nm, 735 nm, and others
• Saturating pulses intensity:
  Blue: Up to 6,000 µmol(photons)/m².s
  Red: Up to 7,500 µmol(photons)/m².s
  White: Up to 5,000 µmol(photons)/m².s
• Actinic light intensity
  Up to 3,000 µmol(photons)/m².s
• Filter wheel
  8 positions
• Light regime
  Static or dynamic (sinus form)
• Actinic and saturating light
  Variable timing, special language and scripts
• Light regime
  Static or dynamic (sinus form)
• Custom defined protocols
  Variable timing, special language and scripts
• CCD detector wavelength range
  400 – 1000 nm
• CCD format
  512 x 512 pixels; optionally: 640 x 480 pixels or 1392 x 1040 pixels
• Pixel size
  8.2 µm x 8.4 µm
• A/D bit resolution
  12 bit
• Spectral response
  QE max at 540 nm (~70 %), 50 % roll-off at 400 nm and 650 nm
• Read-out noise
  Less than 12 electrons RMS - typically only 10 electrons
• Full-well capacity
  Greater than 70,000 electrons (unbinned)
• Imaging frequency
  50 frames per second
• Bios
  Upgradeable firmware
• Communication port
  USB 2.0
• Dimensions
  820 (W) x 820 (D) x 420 (H) mm in FC 800-O/1010
  975 (W) x 975 (D) x 460 (H) mm in FC 800-O/2020
• Weight
  Appr. 35 kg (FC 800-O/1010)
  Appr. 55 kg (FC 800-O/2020)
• Power Input
  Appr. 1100 W (FC 800-O/1010)
  Appr. 1700 W (FC 800-O/2020)
• Electrical
  90 – 240 V

References:

• Berger S. et al. (2006): J. Exp. Bot. 58 (4): 797-806.
• Bartak M. et al. (2006): Ecophysiology 149 (4): 553-560.
• Zabka M. et al. (2006): Mycopathologia 162 (1): 65-68.
• Hajek J., Bartak M. and Dubova J. (2006): Biol. Plant. 50 (4): 624-634.
• Matous K. et al. (2006): Photosynth. Res. 90 (2): 243-253.
• Hogewoning S.W. and Harbinson J. (2007): J. Exp. Bot. 58 (3): 453-463.
• Bartak M. et al. (2007): Polar Biol. 31 (1): 47-51.