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Micro-FluorCam FC 2000

Description of the Micro-FluorCam FC 2000
A: Measuring Flashes; B: Ultra-High Sensitive CCD Camera; C: Supplementary Output;
D: Olympus Frame; E: Variable Excitation Filter Sets; F: Changeable Optical Filter Modules.

Micro-FluorCam FC 2000 extends the complete capacity of kinetic chlorophyll or GFP fluorescence imaging to the realm of individual cells and sub-cellular structures. All conventional fluorescence parameters can be mapped with micrometer resolution so that individual chloroplasts or even grana-stroma thylakoid segments can be investigated.

Key Features:

• Combination of kinetic Chl/GFP-fluorescence imaging and standard wide-field fluorescence microscopy (in certain versions).
• Motorized 6-position filter wheel (in certain versions).
• Multiple fluorophores imaging (in certain versions).
• 10 µs to 20 ms exposure time per frame
• 512 x 512 (or 640 x 480, or 1392 x 1040) pixels spatial resolution.
• 12bit dynamic range.
• 70 % peak quantum yield with about 4 electrons readout noise.
• The instrument combines an attractive price with the advantage of portability.

Scheme of Micro-FluorCam

Oferred Are Five Device Versions:

• Micro-FluorCam FC 2000-ST
• Micro-FluorCam FC 2000-EN
• Micro-FluorCam FC 2000-MFW
• Micro-FluorCam FC 2000-EFW
• Kinetic Fluorescence Microscope FC 2000-Z

1. Micro-FluorCam FC 2000-ST
Included Components: CCD camera; Simple microscope frame; Optical compartment; Control unit; High-performance PC; Excitation light sources; Software package; User's guide.

2. Micro-FluorCam FC 2000-EN
Included Components: CCD camera; Mechanically enhanced microscope frame (Olympus BX40) with exchangeable and extendable components; Mechanically enhanced optical compartment; Control unit; High-performance PC; Excitation light sources; Software package; User's guide.

3. Micro-FluorCam FC 2000-MFW
Included Components: 6-position mechanical filter wheel; CCD camera; Mechanically enhanced microscope frame (Olympus BX40) with exchangeable and extendable components; Mechanically enhanced optical compartment; Control unit; High-performance PC; Excitation light sources; Software package; User's guide.

4. Micro-FluorCam FC 2000-EFW
Included Components: 6-position fully software-controlled filter wheel; CCD camera; Mechanically enhanced microscope frame (Olympus BX40) with exchangeable and extendable components; Mechanically enhanced optical compartment; Control unit; High-performance PC; Excitation light sources; Software package; User's guide.

5. Kinetic Fluorescence Microscope FC 2000-Z
Click here to learn more about the Kinetic Fluorescence Microscope.

All Device Versions Can Be Supplied With:

A. Standard Camera:

• 512 x 512 pixels.
• A/D: 12 bit (4096 grey levels).
• 8.2 µm x 8.4 µm pixel size.
• 50 images per second.
• Advantageous for rapid processes measurements.

B. Advanced Camera:

• 640 x 480 pixels and 1392 x 1040 pixels, respectively.
• A/D: 12 bit (4096 grey levels).
• 6.45 µm x 6.45 µm pixel size.
• 30 and 15 images per second, respectively.
• Great for slow processes measurements and for applications where the higher spatial resolution is of high importance.

Wheat Leaf Image Captured by the Micro-FluorCam

Imaging of Multiple Fluorophores:

• Chlorophyll fluorescence .
• Different fluorescence proteins (GFP, enhanced GFP, blue-emitting variant).
• Other fluorescent dyes (CY3, CY5).

Micro-FluorCam FC2000-EFW

Micro-FluorCam FC2000-EFW: 6-Position Filter Turret (Inset)

References:

• Kuepper H. et al. (2000): Photosyntetica 38 (4): 553-570.
• Ferimazova N. et al. (2002): Photochem. Photobiol. 76, 501-508.
• Vacha F. et al. (2005): Micron 36 (6): 483-502.
• Adamec F., Kaftan D. and Nedbal L. (2005): J. Phycol. 41, p. 835.
• Vacha F. et al. (2007): Micron 38, 170-175.