Handy GFPCam is an easily portable system which can be supplied with a battery charger and small power supply for field experiments. Both the batteries and power supply fit into provided convenient bag. The GFPCam comes with a notebook computer and user-friendly software.
- Combined imaging of GFP and chlorophyll fluorescence
- Both lab and field applications
- Portable and compact (21.5 cm x 13.5 cm x 13.5 cm)
- AC & battery powered
- Coming with a high-end computer and user-friendly software
- High-resolution CCD camera (included)
- Imaged area: 3.5 x 3.5 cm
- Steady-state GFP fluorescence
- FV/FM
- Kautsky induction
- Quenching analysis
- Light curve
- Measured parameters: FO, FM, FV, FO', FM', FV', FT
- More than 50 calculated parameters: FV/FM, FV'/FM', PhiPSII , NPQ, qN, qP, Rfd, PAR-absorptivity coefficient, electron transport rate (ETR), and many others
Images of More Than 50 Calculated Parameters in One Measurement
- Screening for GFP-containing GMOs
- Transgene expression & localization
- Plant-microbe interactions
- Bacterial-protozoan interactions
- Screening for photosynthetic performance
- Stress resistance studies
Arabidopsis Image Captured by the GFPCam:
- GFP mutant expresses in colors
- wildtype expresses in gray (bottom)
- Leaves, small plants, stems, seeds, roots
- Calluses, seedlings on Petri plates
- Bacteria, yeasts, cyanobacteria, green algae
- Small animals
- Sample size up to 3.5 cm x 3.5 cm
Corn Kernel Image
- Homogeneously illuminated area 3.5 cm x 3.5 cm
- Excitation light colors:
- measuring pulses: orange (620 nm), blue (455 nm)
- actinic / saturating light: white – blue (455 nm)
- IR 735 nm - Saturating pulse intensity:
- blue: up to 1,000 µmol(photons)/m².s - Actinic light intensity:
- blue: approx 350 µmol(photons)/m².s - Light regime:
- Static or sinus form (hub connected)
- Progressive scan CCD, 1.4 M pixel sensor
- A/D: 12 bit (4096 grey levels)
- 6.45 µm x 6.45 µm pixel size
- Two operating modes:
- video (ChlF kinetics measurement)
- snapshot (long integration times up to minutes for FPs detection) - Resolution:
- 1392 x 1040 pixels – maximum resolution only in snapshot mode
- 1024 x 768 pixels (10 images per second) – maximum resolution in video mode - Binning option improves sensitivity and provides higher frame rates (both modes):
- 2x2 - 696 x 520 pixels, 25 images per second
- 3x3 – 464 x 344 pixels, 20 images per second
- 4x4 - 348 x 256 pixels, 50 images per second - Intended for combined measurement of ChlF and detection of weak steady-state fluorescence signals with long integration times or applications where higher spatial resolution is of the highest importance (microscopy)
- Fully automated control of the whole FluorCam system
- Image acquisition via automated experimental protocols:
- numerous predefined protocols
- possibility to create user defined protocols via a program Wizard
- multiple (automatically repeated) experiments
- Barcode reader support - Image processing tools:
- automatic or manual image segmentation – e.g., labeling of individual plants
- analysis of kinetic data from all samples within the field of view
- numerous image manipulation tools
- export to text file, avi, bmp or raw data formats - Windows 2000, XP, Vista, W7 compatible*
Corn Kernels Captured by the GFPCam:
- GFP variant in the upper lines
- control kernels in the bottom lines
- Fluorescence Parameters:
- Measured parameters: FO, FM, FV, FO', FM', FV', FT
- More than 50 calculated parameters: FV/FM, FV'/FM', PhiPSII , NPQ, qN, qP, Rfd, PAR-absorptivity coefficient, electron transport rate (ETR), and many others - Excitation Light Sources:
- Measuring pulses: orange (620 nm), blue (455 nm)
- Actinic / saturating light: white - blue (455 nm) - Saturating Pulse Intensity:
Blue: up to 1,000 µmol(photons)/m².s - Actinic Light Intensity:
Blue: approx 350 µmol(photons)/m².s - Light Regime:
Static or sinus form (hub connected) - Custom-Defined Protocols:
Variable timing, special language and scripts - CCD Detector Wavelength Range:
400 – 1000 nm - CCD Format:
512 x 512 pixels; optionally 1024 x 768 pixels or 1392 x 1040 pixels - Pixel Size:
8.2 µm x 8.4 µm; optionally 6.45 µm x 6.45 µm - A/D Bit Resolution:
12 bit - Spectral Response:
QE max at 540 nm (~70 %), 50 % roll-off at 400 nm and 650 nm - Read-out Noise:
Less than 12 electrons RMS - typically only 10 electrons - Full-Well Capacity:
Greater than 70,000 electrons (unbinned) - Imaging Frequency:
Maximum 50 frames per second - Bios:
Upgradeable firmware - Communication Port:
USB 2.0 - GFPCam Weight:
1.8 kg - Leaf-Clip Weight:
0.2 kg - Power Supply Weight:
2.5 kg - GFPCam Stand Weight:
1.5 kg - Notebook Weight (incl. all accessories):
3.5 kg - Power Input:
Max. 200 W - Electrical:
90 – 260 V
Pricing
- Orders and Payments
- Handy GFPCam FC 1000-H/GFP 16.990,- €
- Leaf Clip 390,- €
- Ultra-Light Tripod * 490,- €
- Battery Pack * 490,- €
Optional Features and Accessories
Ultra-Light Tripod
The tripod stand is a very useful accessory for field experiments. Its robust and flexible construction offers needed stability during imaging operations. The stand is water resistant and its variable height allows imaging of samples at different levels within the vegetation. Height up to 1.5 m; weight 3.3 kg; includes a bag.
Leaf Clip
The leaf clip simplifies the process of collecting fluorescence images on vegetation. The unique design includes a gentle lock/release system for holding the target leaf in place without detachment or excessive disturbance. It also allows for dark adaptation.
Battery Pack
A useful supplement for comfortable field measurements. Weight: 3.6 kg. Size: 22 x 20 x 13 cm.
Download
FluorCam 6
OS: Win2000/XP
Language: English
Size: 6.5MB
FluorCam 7
OS: Win XP/Vista/Seven
Language: English
Size: 6.5MB
usb driver for FluorCam
OS: Win2000/XP
Language: English
Size: 6kB
usb driver for FluorCam
OS: Win Vista/Seven
Language: English
Size: 6kB
Documents
FluorCam Handy Brochure
Type: PDF
Language: English
Size: 214kB
FluorCam Leaf-Clip Brochure
Type: PDF
Language: English
Size: 169kB
Micro-FluorCam Brochure
Type: PDF
Language: English
Size: 212kB
Handy GFPCam Brochure
Type: PDF
Language: English
Size: 173kB
FluorCam Manual
Type: PDF
Language: English
Size: 11MB
Driver Installation
Type: PDF
Language: English
Size: 340kB
Other FluorCams
Open FluorCam FC 800-O is a highly modular instrument with flexible geometry. The LED panels and the light source generating saturating flashes can be arranged at various angles and distances from the sample. Also, the position of the camera relative to the sample is adjustable.
Closed FC 800-C
Closed FluorCam FC 800-C represents a highly innovative, world-wide used system of multispectral kinetic fluorescence imaging. It consists of a CCD camera and four fixed LED panels. The LED panels provide uniform irradiance over samples up to 13 x 13 cm – suitable for small plants, detached leaves, algal dilutions, etc. The system allows dark adaptation.
Closed GFPCam FC 800-C/1010GFP
Closed GFPCam FC 800-C/1010GFP serves for various applications using green fluorescent proteins in molecular and cellular biology. The device is equipped with a motorized, software-controlled filter wheel, which provides quick and simple transition between the GFP and chlorophyl fluorescence detection and imaging.
Handy FC 1000-H
Handy FluorCam FC 1000-H is a portable system designed for kinetically resolved fluorescence imaging of leaves and small plants both in a field and laboratory. The whole instrument can be carried in a convenient bag over the shoulder and can run on batteries.
Handy GFPCam FC 1000-H/GFP
Handy GFPCam FC 1000-H/GFP is a modified version of the popular Handy FluorCam. Its unique construction allows both GFP imaging and chlorophyll fluorescence imaging. It can be favorably used for lab and field experiments.
Handy - Leaf Chamber FC 1000-LC
Leaf Chamber Fluorcam FC 1000-LC is a unique system that allows you to conduct chlorophyll fluorescence imaging on a leaf maintained in a leaf chamber, while measuring leaf gas exchange at the same time. The Fluorcam unit can be attached to leaf chambers provided by most manufacturers of leaf gas exchange equipment.
Micro-FluorCam FC 2000
Micro-FluorCam FC 2000 extends the complete capacity of kinetic Chl-fluorescence imaging to the realm of individual cells and sub-cellular structures. It allows investigation of all conventional fluorescence parameters in individual chloroplasts or even grana-stroma thylakoid segments.