Photon Systems Instruments Professional Instruments for Plant Science, Biotechnology, and Agriculture

• Combined imaging of GFP and chlorophyll fluorescence.
• Included high-resolution CCD camera: full resolution 1392 x 1040 (15 fps), 30 fps at resolution 640 x 480; integration times from microseconds to seconds; binning options. The camera is specifically intended for detection of weak signals demanding long integration times; the setup retains most of the functionalities for chlorophyll fluorescence kinetics measurement.
  
• Four light-upgraded LED panels (4 panels 470 nm OR 2 panels 447 nm + 2 panels 470 nm OR 2 panels 627 nm and 2 panels 470 nm) .
• Additional UV, green, yellow, amber, orange or infrared light source (optional).
• 8 position filter wheel - motorized and software-controlled (included).
• Selectable shelf system for different plant sizes (included).
• Dark room for adaptation (included).
• Coming with a high-end computer and user-friendly software (included).
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• Steady-state GFP fluorescence
• Gene transfer and biosensoring.
• Screening for genotype resistance.
• Plant-microbe interactions research.
• Bacterial-protozoan interactions research.
• Agriculture and horticulture.
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  GFP Expression in Corn Kernels (GFPCam Captured)

• Quenching.
• Kautsky induction.
• QA reoxidation (needs optional accessories).
• Fast fluorescence induction (OJIP)(needs optional accessories).
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  Filter Wheel

• Leaves, stems, seeds, roots, whole small plants.
• Calluses, seedlings on petri plates.
• Fruits and vegetables.
• Cyanobacteria, green algae, bacteria.
• Small animals.
• Sample size up to 13 cm x 13 cm.

Proteins of the GFP family demonstrate a great spectral diversity and they often have different excitation and emission spectra. Therefore it is important to use the most appropriate filter sets so as to obtain maximum sensitivity for FP viewing.

• Red-shifted variant ( EGFP)
  The excitation peak is centered at about 488 nm and the emission peak is at 507 - 509 nm.
  Suggested filters: short-pass 480 nm for excitation and 532/28 or 530/25 for emission.
• Original (wild type) GFP variant (wtGPF)
  The major excitation peak is centered at about 395 - 397 nm and the emission peak is at 504 nm.
  Suggested filters: short-pass 420 nm for excitation and 532/28 or 530/25 for emission.
• Blue-emitting variant (BFP)
  The excitation peak at about 384 nm and the emission peak near 448 nm.
  Suggested filters: short-pass 400 nm for excitation and 469/35 for emission.
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  GFPCam Inside View: Camera + LED Panels

• Automated experimental protocols via a Windows Wizard.
• Multiple (automatically repeated) experiments.
• Automated labeling of individual plants, or samples, within the field of view.
• Kinetic analysis of data from all samples within the field of view.
• Numerous image manipulation tools.
• Imaging masks for 384-well plate, 96-well plate, Petri dish, etc.
• Barcode reader support.
• Export to Excel.
• Windows 2000, XP, Vista compatible*.
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  Corn Kernels Captured by the Closed GFPCam:
  - GFP Variant in the Upper Lines
  - Control Kernels in the Bottom Lines