Closed GFPCam FC 800-C/1010GFP
GFP version of the Closed FluorCam can serve for a number of applications using green fluorescent proteins in molecular and cellular biology. The basic system of the Closed FluorCam (CCD camera, four fixed LED panels) is supplemented by a fully motorized and software-controlled filter wheel and appropriate filter sets for the detection and imaging of green fluorescent protein (GFP), red-shifted GFP (EGFP), or other variants of fluorescent proteins. The device configuration also provides user-friendly and quick transition between the GFP and chlorophyll fluorescence detection and imaging.
Applications:
- Gene transfer and biosensoring.
- Screening for genotype resistance.
- Plant-microbe interactions research.
- Bacterial-protozoan interactions research.
- Agriculture and horticulture.
GFP Expression in Corn Kernels (GFPCam Captured)
Experiments and Measured Parameters:
- Quenching.
- Kautsky induction.
- QA reoxidation (needs optional accessories).
- OJIP (needs optional accessories).
- Fast fluorescence induction (needs optional accessories).
- PAR absorptivity (needs optional accessories).
Key Features:
- High-resolution CCD camera: full resolution 1392 x 1040 (15 fps), 30 fps at resolution 640 x 480; integration times from microseconds to seconds; binning options (optional).
Download more information here. - Standard camera: 512 x 512 pixel resolution, 50 fps.
- Up to four super-bright blue LED panels (4 panels 455 nm OR 2 panels 455 nm + 2 panels 475 nm) .
- Additional UV, green, yellow, amber, orange or infrared light source (optional).
- 8 position filter wheel - motorized and software-controlled.
- Selectable shelf system for different plant sizes.
- Dark room for adaptation.
- Coming with a high-end computer and user-friendly software.
GFPCam Inside View: Camera + LED Panels
Experimental Organisms:
- Leaves, stems, seeds, roots, whole small plants.
- Calluses, seedlings on petri plates.
- Fruits and vegetables.
- Cyanobacteria, green algae, bacteria.
- Small animals.
- Sample size up to 13 cm x 13 cm.
Filter Sets for Most Commonly Used GFP Proteins:
Proteins of the GFP family demonstrate a great spectral diversity and they often have different excitation and emission spectra. Therefore it is important to use the most appropriate filter sets so as to obtain maximum sensitivity for FP viewing.
-
Red-shifted variant ( EGFP)
The excitation peak is centered at about 488 nm and the emission peak is at 507 - 509 nm.*
Suggested filters: short-pass 480 nm for excitation and 532/28 or 530/25 for emission. - Original (wild type) GFP variant (wtGPF)
The major excitation peak is centered at about 395 - 397 nm and the emission peak is at 504 nm.*
Suggested filters: short-pass 420 nm for excitation and 532/28 or 530/25 for emission. - Blue-emitting variant (BFP)
The excitation peak at about 384 nm and the emission peak near 448 nm.*
Suggested filters: short-pass 400 nm for excitation and 469/35 for emission.
* Tsien (1998). Annu. Rev. Biochem. 67: 509-544.
Corn Kernels Captured by the Closed GFPCam:
- GFP Variant in the Upper Lines
- Control Kernels in the Bottom Lines
FluorCam 7.0 Software
- Automated experimental protocols via a Windows Wizard.
- Multiple (automatically repeated) experiments.
- Automated labeling of individual plants, or samples, within the field of view.
- Kinetic analysis of data from all samples within the field of view.
- Numerous image manipulation tools.
- Imaging masks for 384-well plate, 96-well plate, Petri dish, etc.
- Barcode reader support.
- Export to Excel.
- Windows 2000, XP, Vista compatible*.
* Windows 2000, Windows XP and Vista are trademarks of Microsoft Corporation.
Add to CartQA-Reoxidation Option - Price: 11.000,- €
Add to CartFast Fluorescence + OJIP Option - Price: 11.000,- €
Add to CartHigh-Resolution Camera - Price: 3.000,- €
Add to Cart8-Position Filter Wheel - Price: 3.490,- €
Add to CartInfrared Light Source (13 x 13 cm) - Price: 1.990,- €
Add to CartSoftware Module for Barcode Reader - Price: 910,- €
Add to CartOptional Features and Accessories:
QA-Reoxidation Option
High-end option allowing determination of QA-reoxidation kinetics. QA-reoxidation is recorded in a pump-and-probe manner, i.e. using repetitive flashes and recording with variable delay after each flash.
Fast Fluorescence + OJIP Option
Special electronic module for tracking fast fluorescence and OJIP. The processes are recorded in multiple light exposures, each with a variable delay after the light is switched on.
High-Resolution Camera
Ultra high-performance camera with fast global shutter has two fast frame rates: 30 fps at 640 x 480 pixels or 15 fps at 1392 x 1040 pixels. Great for slow processes measurements and for applications where the higher spatial resolution is of high importance.
Download more information here.
Filter Wheel
8-position software-controlled filter wheel allows for imaging in different emission bands. Filters are easily interchangeable.
Infrared Light Source (735 nm)
Additional light source that enables to determine Fo' and PAR absorbance (when accompanied with filter wheel). Size: 13 x 13 cm.
Software Module for Barcode Reader
Program module for a fully automatic bar code search.
