- FV/FM
- Kautsky induction
- Quenching analysis
- Steady state fluorescence, e.g., ChlF, GFP and other FPs (filter wheel required)
- Multicolor fluorescence, e.g., ChlF, plant blue-green fluorescence (filter wheel required)
- QA re-oxidation (needs optional electronic module)
- Fast fluorescence induction (OJIP) with 1µs resolution (needs optional electronic module)
- PAR absorptivity (needs optional accessories: filter wheel and additional IR LED panel)
- Measured parameters: FO, FM, FV, FO', FM', FV', FT
- More than 50 calculated parameters: FV/FM, FV'/FM', PhiPSII , NPQ, qN, qP, Rfd, PAR-absorptivity coefficient, electron transport rate (ETR), and many others
Images of More Than 50 Calculated Parameters in One Measurement
- Screening for photosynthetic performance and metabolic perturbations
- Detection of biotic and abiotic stress
- Plant’s resistance or susceptibility to various stress factors
- Stomatal patchiness
- Yield improvement
- Growth and development
- Leaves, small plants, fruits, vegetables
- Mosses, lichens
- Cyanobacteria, green algae
- Sample size up to 13 cm x 13 cm
- Selectable shelf system for different plant size
- Predefined imaging masks for 384-well plate, 96-well plate, Petri dish, etc.
- Dark room for adaptation
- Standard version of the FluorCam system includes four super bright LED panels. One pair of LED panels provides Measuring Light and Actinic Light 1 (typically 618 nm). Two panels provide Actinic Light 2 and Saturating Pulse (these two panels are mounted on customer's demand in blue (455 nm), red (618 nm), or white color)
- Other available wavelengths are: 470 nm, 505 nm, 570 nm, 605 nm, 630 nm (in customized FluorCam versions)
- Additional LED panel may be mounted round the camera to the top stand in infra-red (735 nm), ultra-violet (360 or 380 nm), or green (505 nm) version (optional)
- Actinic light intensity:
- 300-2,500 µmol(photons)/m².s in the standard version depending on light wavelength,
- up to 5,000 µmol(photons)/m².s in the light-upgraded version - please inquire at: zc.isp@ofni - Super pulse intensity:
- up to 3,000 µmol(photons)/m².s (in the standard version),
- up to 10,000 µmol(photons)/m².s (in the light-upgraded version - please inquire at: zc.isp@ofni - Single Turnover Flash (STF): 120,000 µmol(photons)/m².s in 100µs pulse (in the QA re-oxidation version)
- Comparison of light intensities reachable by the standard and light upgraded (QA) panels:
FluorCam FC-800 - Standard Version Light Type Actinic 1 Color red-orange Wavelength [nm] 617 Intensity [umol] 200-300 Light Type Actinic 2 Color red-orange royal-blue cool white neutral white warm white Wavelength [nm] 617 447 4500 - 10000 K 3500 - 4500 K 2540 - 3500 K Intensity [umol] 2000 2000 2000 Light Type Saturating Pulse Color red-orange royal-blue cool white neutral white warm white Wavelength [nm] 617 447 4500 - 10000 K 3500 - 4500 K 2540 - 3500 K Intensity [umol] 4000 4000 4000
FluorCam FC-800 - Light Upgraded Version / QA Version Light Type Actinic 1 Color red-orange Wavelength [nm] 617 Intensity [umol] 2000 Light Type Actinic 2 Color red-orange royal-blue cool white neutral white warm white Wavelength [nm] 617 447 4500 - 10000 K 3500 - 4500 K 2540 - 3500 K Intensity [umol] 3000 3000 3000 Max Intensity [umol] 8000 8000 9000 Light Type Saturating Pulse Color red-orange royal-blue cool white neutral white warm white Wavelength [nm] 617 447 4500 - 10000 K 3500 - 4500 K 2540 - 3500 K Intensity [umol] 6000 6000 6000 Max Intensity [umol] 16000 16000 16000
- 8 position filter wheel (optional)
- Chlorophyll fluorescence (high pass 695 nm, low pass 780 nm), GFP (high pass 495, low pass 660 nm, band pass 505/560 nm), PAR (clear glass), YFP, CY3, CY5, as well as other fluorescence colors
- See also: Closed FluorCam GFP Version for detection and imaging of GFP, red-shifted GFP, or blue-emitting protein variants
- 512 x 512 pixels
- A/D: 12 bit (4096 grey levels)
- 8.2 µm x 8.4 µm pixel size
- 50 images per second
- Advantageous for rapid processes measurements
- Progressive scan CCD, 1.4 M pixel sensor
- A/D: 12 bit (4096 grey levels)
- 6.45 µm x 6.45 µm pixel size
- Two operating modes:
- video (ChlF kinetics measurement),
- snapshot (long integration times up to minutes for FPs detection) - Resolution:
- 1392 x 1040 pixels – maximum resolution only in snapshot mode,
- 1024 x 768 pixels (10 images per second) – maximum resolution in video mode - Binning option improves sensitivity and provides higher frame rates (both modes):
- 2x2 - 696 x 520 pixels, 25 images per second,
- 3x3 – 464 x 344 pixels, 20 images per second,
- 4x4 - 348 x 256 pixels, 50 images per second - Intended for combined measurement of ChlF and detection of weak steady-state fluorescence signals with long integration times or applications where the higher spatial resolution is of highest importance (microscopy)
- Fully automated control of the whole FluorCam system
- Image acquisition through automated experimental protocols:
- numerous predefined protocols,
- possibility to create user defined protocols via a Windows Wizard,
- multiple (automatically repeated) experiments,
- Barcode reader support - Image processing tools:
- automatic or manual image segmentation – e.g., labeling of individual plants,
- analysis of kinetic data from all samples within the field of view,
- numerous image manipulation tools,
- export to text file, avi, bmp or raw data formats - Windows 2000, XP, Vista, W7 compatible*
FluorCam Software Window
- Fluorescence Parameters:
- Measured parameters: FO, FM, FV, FO', FM', FV', FT
- More than 50 calculated parameters: FV/FM, FV'/FM', PhiPSII , NPQ, qN, qP, Rfd, PAR-absorptivity coefficient, electron transport rate (ETR), and many others - Light Sources:
455 nm, 470 nm, 505 nm, 570 nm, 605 nm, 618 nm, 630 nm, 735 nm, white and others - Super Pulse Intensity:
- 3,000 µmol(photons)/m².s (in a standard version)
- 10,000 µmol(photons)/m².s (in the light-upgraded version) - Actinic Light Intensity:
Up to 2,500 µmol(photons)/m².s (depending on light wavelength) - Filter Wheel:
8 positions - Light Regime:
Static or dynamic (sinus form) - Custom-Defined Protocols:
Variable timing, special language and scripts - CCD Detector Wavelength Range:
400 – 1000 nm - CCD Format:
512 x 512 pixels; optionally 1024 x 768 pixels or 1392 x 1040 pixels - Pixel Size:
8.2 µm x 8.4 µm; optionally 6.45 µm x 6.45 µm - A/D bit Resolution:
12 bit - Spectral Response:
QE max at 540 nm (~70 %), 50 % roll-off at 400 nm and 650 nm - Read-Out Noise:
Less than 12 electrons RMS - typically only 10 electrons - Full-Well Capacity:
Greater than 70,000 electrons (unbinned) - Imaging Frequency:
Maximum 50 frames per second - Bios:
Upgradeable firmware - Communication Port:
USB 2.0 - Dimension:
471 mm (W) x 473 mm (D) x 512 mm (H) - Weight:
Appr. 40 kg - Power Input:
Appr. 1100 W - Electrical:
90 – 240 V
- Chi W. et al. (2008): Plant Physiol. 147: 573-584.
- Miura E. et al. (2007): Plant Cell 19: 1313-1328.
- Hogewoning S.W. and Harbinson J. (2007): J. Exp. Bot. 58 (3): 453-463.
- Bartak M. et al. (2007): Polar Biol. 31 (1): 47-51.
- Pfalz J. et al. (2006): Plant Cell 18: 176-197.
- Berger S. et al. (2006): J. Exp. Bot. 58 (4): 797-806.
- Bartak M. et al. (2006): Ecophysiology 149 (4): 553-560.
- Zabka M. et al. (2006): Mycopathologia 162 (1): 65-68.
- Hajek J., Bartak M. and Dubova J. (2006): Biol. Plant. 50 (4): 624-634.
- Matous K. et al. (2006): Photosynth. Res. 90 (2): 243-253.
- Diaz C. et al. (2005): Plant Physiology 138: 898-908.
Pricing
- Orders and Payments
- Closed FluorCam FC 800-C/1010 13.990,- €
- Additional Infra-Red Light Panel 1.990,- €
- Additional Ultraviolet Light Panel 2.990,- €
- Additional Cyan/Blue/Green/Yellow Light Panel 2.490,- €
- High-Resolution Camera * 3.000,- €
- 8-Position Filter Wheel 3.490,- €
- QA-Reoxidation Module 11.000,- €
- PAR Absorptivity Module 5.480,- €
- Fast Fluorescence Induction (OJIP) Module Inquire for price,- €
- Software Module for Barcode Reader 910,- €
Optional Features and Accessories
QA-Reoxidation Module
High-end option allowing determination of QA-reoxidation kinetics. QA-reoxidation is recorded in a pump-and-probe manner, i.e. using repetitive flashes and recording with variable delay after each flash.
PAR-Absorptivity Module
High-end option for measuring PAR absorptivity includes an 8-position filter wheel plus infra-red light source 13 x 13 cm.
Fast Fluorescence Induction (OJIP) Module
Special electronic module for tracking fast fluorescence induction - OJIP. The processes are recorded in multiple light exposures, each with a variable delay after the light is switched on.
High-Resolution Camera
High-performance camera with 1.4 M pixel sensor and 6.45 µm x 6.45 µm pixel size. Great for combined measurement of ChlF and detection of weak steady-state fluorescence signals with long integration times or applications where the higher spatial resolution is of highest importance (microscopy).
Filter Wheel
8-position software-controlled filter wheel allows for imaging in different emission bands. Filters are easily interchangeable.
Additional LED Light Panels
Additional LED light panel is mounted at the top part of the FluorCam System. It is located under the camera; the objective of the camera is inserted in the central opening of this panel. The panel widens the possibility of employing more excitation colors for special applications. Available panel versions: ultraviolet, blue, cyan, green, yellow, orange, or infra-red.
Ultraviolet Panel - Recommended use: (1) multicolor fluorescence - UV excited blue F440, green F520, red F680, and infra-red F740 (must be accompanied with the filter wheel and high-resolution CCD); (2) excitation source for wtGFP, DAPI (filter wheel and high-resolution CCD required).
Cyan Panel: Recommended use: (1) studies on stomata function; (2) excitation source for EGFP (must be accompanied with the filter wheel and high-resolution CCD).
Green Panel: Recommended use: excitation source for YFP (must be accompanied with the filter wheel and high-resolution CCD).
Infra-Red Panel: Recommended use: determination of Fo' and PAR absorbance (use of filter wheel required).
Software Module for Barcode Reader
Program module for a fully automatic bar code search.
Download
FluorCam 6
OS: Win2000/XP
Language: English
Size: 6.5MB
FluorCam 7
OS: Win XP/Vista/Seven
Language: English
Size: 6.5MB
usb driver for FluorCam
OS: Win2000/XP
Language: English
Size: 6kB
usb driver for FluorCam
OS: Win Vista/Seven
Language: English
Size: 6kB
Documents
FluorCam Handy Brochure
Type: PDF
Language: English
Size: 214kB
FluorCam Leaf-Clip Brochure
Type: PDF
Language: English
Size: 169kB
Micro-FluorCam Brochure
Type: PDF
Language: English
Size: 212kB
Handy GFPCam Brochure
Type: PDF
Language: English
Size: 173kB
FluorCam Manual
Type: PDF
Language: English
Size: 11MB
Driver Installation
Type: PDF
Language: English
Size: 340kB
Other FluorCams
Open FluorCam FC 800-O is a highly modular instrument with flexible geometry. The LED panels and the light source generating saturating flashes can be arranged at various angles and distances from the sample. Also, the position of the camera relative to the sample is adjustable.
Closed FC 800-C
Closed FluorCam FC 800-C represents a highly innovative, world-wide used system of multispectral kinetic fluorescence imaging. It consists of a CCD camera and four fixed LED panels. The LED panels provide uniform irradiance over samples up to 13 x 13 cm – suitable for small plants, detached leaves, algal dilutions, etc. The system allows dark adaptation.
Closed GFPCam FC 800-C/1010GFP
Closed GFPCam FC 800-C/1010GFP serves for various applications using green fluorescent proteins in molecular and cellular biology. The device is equipped with a motorized, software-controlled filter wheel, which provides quick and simple transition between the GFP and chlorophyl fluorescence detection and imaging.
Handy FC 1000-H
Handy FluorCam FC 1000-H is a portable system designed for kinetically resolved fluorescence imaging of leaves and small plants both in a field and laboratory. The whole instrument can be carried in a convenient bag over the shoulder and can run on batteries.
Handy GFPCam FC 1000-H/GFP
Handy GFPCam FC 1000-H/GFP is a modified version of the popular Handy FluorCam. Its unique construction allows both GFP imaging and chlorophyll fluorescence imaging. It can be favorably used for lab and field experiments.
Handy - Leaf Chamber FC 1000-LC
Leaf Chamber Fluorcam FC 1000-LC is a unique system that allows you to conduct chlorophyll fluorescence imaging on a leaf maintained in a leaf chamber, while measuring leaf gas exchange at the same time. The Fluorcam unit can be attached to leaf chambers provided by most manufacturers of leaf gas exchange equipment.
Micro-FluorCam FC 2000
Micro-FluorCam FC 2000 extends the complete capacity of kinetic Chl-fluorescence imaging to the realm of individual cells and sub-cellular structures. It allows investigation of all conventional fluorescence parameters in individual chloroplasts or even grana-stroma thylakoid segments.