Closed FluorCam FC 800-C

Closed FluorCamClosed FluorCam represents a highly innovative, world-wide used system of multispectral kinetic fluorescence imaging. It consists of a CCD camera, four fixed LED panels and, optionally, of an 8-position filter wheel. The LED panels provide uniform irradiance over samples up to 13 x 13 cm – suitable for small plants, detached leaves, algal dilutions, etc. The system allows dark adaptation. The price includes a high-performance PC and comprehensive software package.

Experiments and Measured Parameters:

  • Quenching.
  • Kautsky induction.
  • QA reoxidation (needs optional accessories).
  • OJIP (needs optional accessories).
  • Fast fluorescence induction with 1µs resolution (needs optional accessories).
  • PAR absorptivity (needs optional accessories).
  • Measured parameters: Fo, FM, FV, Fo’, FM’, FV’, QY(II).
  • More than 50 calculated parameters: NPQ, FV/FM, FV’/FM’, Rfd, qN, qP, PAR-absorptivity, electron transport rate (ETR), and many others.

Calculated parameters
Images of More Than 50 Calculated Parameters in One Measurement

Applications:

  • Screening for photosynthetic performance.
  • Stress resistance or susceptibility.
  • Stomatal patchiness.
  • Metabolic perturbations.
  • Growth and yield.

Samples:

  • Leaves, plants, fruits, vegetables.
  • Cyanobacteria, green algae.
  • Sample size up to 13 cm x 13 cm.
  • Imaging masks for 384-well plate, 96-well plate, Petri dish, etc.
  • Selectable shelf system for different plant sizes.
  • Dark room for adaptation.

Light Sources:

  • Actinic light up to 3,000 µmol(photons)/m².s.
  • Super pulse up to 7,500 µmol(photons)/m².s.
  • STF - Single Turnover Flash.
  • IR 735 nm (FAR).
  • The FluorCam system includes four super bright LED panels.
  • Two panels provide Measuring Light and Actinic Light 1 (red light 618 nm in a standard version). Two panels provide Actinic Light 2 and Saturating Pulse ( these two panels are mounted according to customer's needs in blue (455 nm), red (618 nm), or white color).
  • Other wavelengths are available as option: 390 nm, 470 nm, 505 nm, 570 nm, 605 nm, 630 nm, 735 nm, and others.
  • Additional light sources, mounted close to the camera stand or to the device side, may be provided, e.g., infra-red, ultra-violet, or green (optional).
  • Variable excitation color.

Different Emission Bands:

  • 8 position filter wheel (optional).
  • Chlorophyll fluorescence (high pass 695 nm, low pass 780 nm), GFP (high pass 495, low pass 660 nm, band pass 505/560 nm), PAR (clear glass), YFP, CY3, CY5, as well as other fluorescence colors.
  • COMPLETELY NEW!!!
    See: Closed FluorCam GFP Version for detection and imaging of GFP, red-shifted GFP, or blue-emitting protein variants.

Standard Imaging Format:

  • 512 x 512 pixels.
  • A/D: 12 bit (4096 grey levels).
  • 8.2 µm x 8.4 µm pixel size.
  • 50 images per second.
  • Advantageous for rapid processes measurements.

Optional Imaging Format:

  • 640 x 480 pixels and 1392 x 1040 pixels, respectively.
  • A/D: 12 bit (4096 grey levels).
  • 6.45 µm x 6.45 µm pixel size.
  • 30 and 15 images per second, respectively.
  • Great for slow processes measurements and for applications where the higher spatial resolution is of high importance.

FluorCam Software Window

NEW FluorCam 7.0 Software

  • Automated experimental protocols via a Windows Wizard.
  • Multiple (automatically repeated) experiments.
  • Automated labeling of individual plants, or samples, within the field of view.
  • Kinetic analysis of data from all samples within the field of view.
  • Numerous image manipulation tools.
  • Barcode reader support.
  • Export to Excel.
  • Windows 2000, XP, Vista compatible*.

Technical Specification:

  • Fluorescence parameters
    (F0, FM, FV, F0’, FM’, FV’, QY(II)), Abs PAR-value, or the parameters that are calculated from fluorescence emission (e.g., NPQ, FV/FM, FV’/FM’, Rfd, qN, qP), PAR-absorptivity, photosynthetic electron transport rate (PS), and others
  • Excitation light sources
    360 nm, 390 nm, 450 nm, 470 nm, 505 nm, 570 nm, 605 nm, 630 nm, 735 nm, and others
  • Saturating pulses intensity:
    Blue: Up to 6,000 µmol(photons)/m².s
    Red: Up to 7,500 µmol(photons)/m².s
    White: Up to 5,000 µmol(photons)/m².s
  • Actinic light intensity
    Up to 3,000 µmol(photons)/m².s
  • Filter wheel
    8 positions
  • Light regime
    Static or dynamic (sinus form)
  • Actinic and saturating light
    Variable timing, special language and scripts
  • Light regime
    Static or dynamic (sinus form)
  • Custom defined protocols
    Variable timing, special language and scripts
  • CCD detector wavelength range
    400 – 1000 nm
  • CCD format
    512 x 512, 640 x 480, 1392 x 1040 pixels
  • A/D bit resolution
    12 bit
  • Spectral response
    QE max at 540 nm (~70 %), 50 % roll-off at 400 nm and 650 nm
  • Read-out noise
    Less than 12 electrons RMS - typically only 10 electrons
  • Full-well capacity
    Greater than 70,000 electrons (unbinned)
  • Imaging frequency
    50 frames per second
  • Bios
    Upgradeable firmware
  • Communication port
    USB 2.0
  • Dimensions
    480 x mm x 530 mm
  • Weight
    40 kg
  • Electrical
    90 – 240 V

* Windows 2000, Windows XP and Vista are trademarks of Microsoft Corporation.

 

References:

  • Chi W. et al. (2008): Plant Physiol. 147: 573-584.
  • Miura E. et al. (2007): Plant Cell 19: 1313-1328.
  • Hogewoning S.W. and Harbinson J. (2007): J. Exp. Bot. 58 (3): 453-463.
  • Bartak M. et al. (2007): Polar Biol. 31 (1): 47-51.
  • Pfalz J. et al. (2006): Plant Cell 18: 176-197.
  • Berger S. et al. (2006): J. Exp. Bot. 58 (4): 797-806.
  • Bartak M. et al. (2006): Ecophysiology 149 (4): 553-560.
  • Zabka M. et al. (2006): Mycopathologia 162 (1): 65-68.
  • Hajek J., Bartak M. and Dubova J. (2006): Biol. Plant. 50 (4): 624-634.
  • Matous K. et al. (2006): Photosynth. Res. 90 (2): 243-253.
  • Diaz C. et al. (2005): Plant Physiology 138: 898-908.

 

Closed FC 800-C - Price: 13.990,- €
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QA-Reoxidation Option - Price: 11.000,- €
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PAR Absorptivity Option - Price: 5.480,- €
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Fast Fluorescence + OJIP Option - Price: 11.000,- €
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Advanced Camera - Price: 3.000,- €
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8-Position Filter Wheel - Price: 3.490,- €
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Infrared Light Source (13 x 13 cm) - Price: 1.990,- €
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Software Module for Barcode Reader - Price: 910,- €
Add to Cart

Optional Features and Accessories:

QA-Reoxidation Option
High-end option allowing determination of QA-reoxidation kinetics. QA-reoxidation is recorded in a pump-and-probe manner, i.e. using repetitive flashes and recording with variable delay after each flash.

PAR-Absorptivity Option
High-end option for measuring PAR absorptivity includes an 8-position filter wheel plus infra-red light source 13 x 13 cm.

Fast Fluorescence + OJIP Option
Special electronic module for tracking fast fluorescence and OJIP. The processes are recorded in multiple light exposures, each with a variable delay after the light is switched on.

Advanced High-Resolution Camera
Ultra high-performance camera with fast global shutter has two fast frame rates: 30 fps at 640 x 480 pixels or 15 fps at 1392 x 1040 pixels. Great for slow processes measurements and for applications where the higher spatial resolution is of high importance.

Filter Wheel
8-position software-controlled filter wheel allows for imaging in different emission bands. Filters are easily interchangeable.

Infrared Light Source (735 nm)
Additional light source that enables to determine Fo' and PAR absorbance (when accompanied with filter wheel). Size: 13 x 13 cm.

Software Module for Barcode Reader
Program module for a fully automatic bar code search.

Download Software
Download Documents
Support - your comments or questions
Other FluorCams:

Open FC 800-O/1010
Closed GFPCam FC 800-C/1010GFP
Handy FC 1000-H
Handy GFPCam FC 1000-H/GFP
Handy - Leaf Chamber FC 1000-LC
Micro-FluorCam FC 2000
Video:

Temperature Video - High Temperature Exposure in Soy Leaf (MPG 0.9MB)

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