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Closed FluorCam FC 800-C

Closed FluorCam represents a highly innovative, world-wide used system of multispectral kinetic fluorescence imaging. It consists of a CCD camera, four fixed LED panels and, optionally, of an 8-position filter wheel. The LED panels provide uniform irradiance over samples up to 13 x 13 cm – suitable for small plants, detached leaves, algal dilutions, etc. The system allows dark adaptation. The price includes a high-performance PC and comprehensive software package.

Experiments and Measured Parameters:

• Quenching.
• Kautsky induction.
• QA reoxidation (needs optional accessories).
• OJIP (needs optional accessories).
• Fast fluorescence induction with 1µs resolution (needs optional accessories).
• PAR absorptivity (needs optional accessories).
• Measured parameters: Fo, FM, FV, Fo’, FM’, FV’, QY(II).
• More than 50 calculated parameters: NPQ, FV/FM, FV’/FM’, Rfd, qN, qP, PAR-absorptivity, electron transport rate (ETR), and many others.

Images of More Than 50 Calculated Parameters in One Measurement

Applications:

• Screening for photosynthetic performance.
• Stress resistance or susceptibility.
• Stomatal patchiness.
• Metabolic perturbations.
• Growth and yield.

Samples:

• Leaves, plants, fruits, vegetables.
• Cyanobacteria, green algae.
• Sample size up to 13 cm x 13 cm.
• Imaging masks for 384-well plate, 96-well plate, Petri dish, etc.
• Selectable shelf system for different plant sizes.
• Dark room for adaptation.

Light Sources:

• Actinic light up to 3,000 µmol(photons)/m².s.
• Super pulse up to 7,500 µmol(photons)/m².s.
• STF - Single Turnover Flash.
• IR 735 nm (FAR).
• The FluorCam system includes four super bright LED panels.
• Two panels provide Measuring Light and Actinic Light 1 (red light 618 nm in a standard version). Two panels provide Actinic Light 2 and Saturating Pulse ( these two panels are mounted according to customer's needs in blue (455 nm), red (618 nm), or white color).
• Other wavelengths are available as option: 390 nm, 470 nm, 505 nm, 570 nm, 605 nm, 630 nm, 735 nm, and others.
• Additional light sources, mounted close to the camera stand or to the device side, may be provided, e.g., infra-red, ultra-violet, or green (optional).
• Variable excitation color.

Different Emission Bands:

• 8 position filter wheel (optional).
• Chlorophyll fluorescence (high pass 695 nm, low pass 780 nm), GFP (high pass 495, low pass 660 nm, band pass 505/560 nm), PAR (clear glass), YFP, CY3, CY5, as well as other fluorescence colors.
• COMPLETELY NEW!!!
  See: Closed FluorCam GFP Version for detection and imaging of GFP, red-shifted GFP, or blue-emitting protein variants.

Standard Imaging Format:

• 512 x 512 pixels.
• A/D: 12 bit (4096 grey levels).
• 8.2 µm x 8.4 µm pixel size.
• 50 images per second.
• Advantageous for rapid processes measurements.

Optional Imaging Format:

• 640 x 480 pixels and 1392 x 1040 pixels, respectively.
• A/D: 12 bit (4096 grey levels).
• 6.45 µm x 6.45 µm pixel size.
• 30 and 15 images per second, respectively.
• Great for slow processes measurements and for applications where the higher spatial resolution is of high importance.

NEW FluorCam 7.0 Software

• Automated experimental protocols via a Windows Wizard.
• Multiple (automatically repeated) experiments.
• Automated labeling of individual plants, or samples, within the field of view.
• Kinetic analysis of data from all samples within the field of view.
• Numerous image manipulation tools.
• Barcode reader support.
• Export to Excel.
• Windows 2000, XP, Vista compatible*.

Technical Specification:

• Fluorescence parameters
  (F0, FM, FV, F0’, FM’, FV’, QY(II)), Abs PAR-value, or the parameters that are calculated from fluorescence emission (e.g., NPQ, FV/FM, FV’/FM’, Rfd, qN, qP), PAR-absorptivity, photosynthetic electron transport rate (PS), and others
• Excitation light sources
  360 nm, 390 nm, 450 nm, 470 nm, 505 nm, 570 nm, 605 nm, 630 nm, 735 nm, and others
• Saturating pulses intensity:
  Blue: Up to 6,000 µmol(photons)/m².s
  Red: Up to 7,500 µmol(photons)/m².s
  White: Up to 5,000 µmol(photons)/m².s
• Actinic light intensity
  Up to 3,000 µmol(photons)/m².s
• Filter wheel
  8 positions
• Light regime
  Static or dynamic (sinus form)
• Actinic and saturating light
  Variable timing, special language and scripts
• Light regime
  Static or dynamic (sinus form)
• Custom defined protocols
  Variable timing, special language and scripts
• CCD detector wavelength range
  400 – 1000 nm
• CCD format
  512 x 512, 640 x 480, 1392 x 1040 pixels
• A/D bit resolution
  12 bit
• Spectral response
  QE max at 540 nm (~70 %), 50 % roll-off at 400 nm and 650 nm
• Read-out noise
  Less than 12 electrons RMS - typically only 10 electrons
• Full-well capacity
  Greater than 70,000 electrons (unbinned)
• Imaging frequency
  50 frames per second
• Bios
  Upgradeable firmware
• Communication port
  USB 2.0
• Dimensions
  480 x mm x 530 mm
• Weight
  40 kg
• Electrical
  90 – 240 V

* Windows 2000, Windows XP and Vista are trademarks of Microsoft Corporation.

References:

• Chi W. et al. (2008): Plant Physiol. 147: 573-584.
• Miura E. et al. (2007): Plant Cell 19: 1313-1328.
• Hogewoning S.W. and Harbinson J. (2007): J. Exp. Bot. 58 (3): 453-463.
• Bartak M. et al. (2007): Polar Biol. 31 (1): 47-51.
• Pfalz J. et al. (2006): Plant Cell 18: 176-197.
• Berger S. et al. (2006): J. Exp. Bot. 58 (4): 797-806.
• Bartak M. et al. (2006): Ecophysiology 149 (4): 553-560.
• Zabka M. et al. (2006): Mycopathologia 162 (1): 65-68.
• Hajek J., Bartak M. and Dubova J. (2006): Biol. Plant. 50 (4): 624-634.
• Matous K. et al. (2006): Photosynth. Res. 90 (2): 243-253.
• Diaz C. et al. (2005): Plant Physiology 138: 898-908.