FLUORCAMS

Open FC 800-O/1010 NEW

Open FluorCam FC 800-O is a highly modular instrument with flexible geometry. The LED panels and the light source generating saturating flashes can be arranged at various angles and distances from the sample. Also, the position of the camera relative to the sample is adjustable.

Closed FC 800-C

Closed FluorCam FC 800-C represents a highly innovative, world-wide used system of multispectral kinetic fluorescence imaging. It consists of a CCD camera and four fixed LED panels. The LED panels provide uniform irradiance over samples up to 13 x 13 cm – suitable for small plants, detached leaves, algal dilutions, etc. The system allows dark adaptation.

Closed GFPCam NEW

Closed GFPCam FC 800-C/1010GFP serves for various applications using green fluorescent proteins in molecular and cellular biology. The device is equipped with a motorized, software-controlled filter wheel, which provides quick and simple transition between the GFP and chlorophyl fluorescence detection and imaging.

Handy FC 1000-H

Handy FluorCam FC 1000-H is a portable system designed for kinetically resolved fluorescence imaging of leaves and small plants both in a field and laboratory. The whole instrument can be carried in a convenient bag over the shoulder and can run on batteries.

Handy GFPCam

Handy GFPCam FC 1000-H/GFP is a modified version of the HandyCam. It is primarily used for imaging of fluorescence protein emission in samples with dimensions ranging from millimeters to centimeters.

Handy - Leaf Chamber FC 1000-LC

Leaf Chamber Fluorcam FC 1000-LC is a unique system that allows you to conduct chlorophyll fluorescence imaging on a leaf maintained in a leaf chamber, while measuring leaf gas exchange at the same time. The Fluorcam unit can be attached to leaf chambers provided by most manufacturers of leaf gas exchange equipment.

Micro-FluorCam FC 2000

Micro-FluorCam FC 2000 extends the complete capacity of kinetic Chl-fluorescence imaging to the realm of individual cells and sub-cellular structures. It allows investigation of all conventional fluorescence parameters in individual chloroplasts or even grana-stroma thylakoid segments.

FluorCams are imaging fluorometers that measure sequences of Chl-fluorescence images with user-defined irradiance protocols and timing of measurements. Fluorescence emission is induced by two (or more) panels of super-bright, light emitting diodes (LEDs) that provide measuring flashes. Photochemistry is driven by continuous actinic irradiance and/or by strong saturating pulses of light that are generated in a halogen lamp, equipped with a shutter, or in supplemental LED panels.

Fluorescence images are captured by a CCD camera. The images are taken at 12-bit resolution in 512 x 512 pixels (or 640 x 480 or 1392 x 1040 pixels) of a CCD chip. Up to fifty images can be taken in one second. Data are transmitted via a USB 2.0 port to a desktop computer or to a notebook.

The software package includes numerous image manipulation tools and also a Wizard with the most frequently used experimental protocols. For an experienced professional, the software offers a sophisticated programming language that can be used for designing novel timing and measurig sequences.

Typically, FluorCam is used to detect dynamics and spatial heterogeneity in fluorescence emission of plants. Kautsky effect, quenching analysis, and other transients are imaged and 2-dimensional maps - images of conventional fluorescence parameters - are calculated. Images of F₀, F_M, F_V, F₀', F_M', F_V', NPQ, Φ_PSII, F_V/F_M, F_V'/F_M', R_Fd, q_N, q_P, and of other fluorescence parameters are used, for example, to investigate heterogeneity due to infection, senescence, abiotic stress, or mutation.

Measured objects can be as small as isolated chloroplasts or individual cells in the Micro-FluorCam and Fluorescence Kinetic Microscope or as large as small canopies in the Arch, Transect, or Rover FluorCams. In addition to standard imaging of chlorophyll fluorescence emission, one can use the FluorCam to image fluorescence emission of all varieties of fluorescent proteins - Handy GFPCam or Closed GFPCam.