CUSTOMIZED FLUORCAMS

Fluorescence Kinetic Microscope FC 2000-Z | XY-Plane FC 900-XY/8040 | Transect FC 900-TR | Rover FC 900-R | Arch FC 900-A

Fluorescence Kinetic Microscope FC 2000-Z

Fluorescence Kinetic Microscope is the most versatile tool for lab-based research. It allows measurements with various user-selectable excitation and emission wavelengths as well as the combination of imaging measurements with spectrally resolved or ultra fast spot-measurements of fluorescence and absorbance kinetics.

XY-Plane FC 900-XY/8040 NEW

XY-Plane is a user-friendly fluorescence imaging system that has been optimized for high throughput screening and multispectral analysis of multiple samples in large growth trays.

Transect FC 900-TR

Transect FluorCam is an open fluorescence imaging system for large-scale screening in laboratory or scanning in field. It scans plants along a two meters long transect.

Rover FC 900-R NEW

Rover FluorCam is a customized fluorescence imaging system for large-scale scanning in field. It is a remarkably stable structure, yet its large and solid wheels allow easy movement. The system allows dark adaptation.

Arch FC 900-A

Arch FluorCam is a customized fluorescence imaging system for three-dimensional studies. Large plants can be analyzed from various positions without the need to move them.

FluorCams are imaging fluorometers that measure sequences of Chl-fluorescence images with user-defined irradiance protocols and timing of measurements. Fluorescence emission is induced by two (or more) panels of super-bright, light emitting diodes (LEDs) that provide measuring flashes. Photochemistry is driven by continuous actinic irradiance and/or by strong saturating pulses of light that are generated in a halogen lamp, equipped with a shutter, or in supplemental LED panels.

Fluorescence images are captured by a CCD camera. The images are taken at 12-bit resolution in 512 x 512 pixels (or 640 x 480 or 1392 x 1040 pixels) of a CCD chip. Up to fifty images can be taken in one second. Data are transmitted via a USB 2.0 port to a desktop computer or to a notebook.

The software package includes numerous image manipulation tools and also a Wizard with the most frequently used experimental protocols. For an experienced professional, the software offers a sophisticated programming language that can be used for designing novel timing and measurig sequences.

Typically, FluorCam is used to detect dynamics and spatial heterogeneity in fluorescence emission of plants. Kautsky effect, quenching analysis, and other transients are imaged and 2-dimensional maps - images of conventional fluorescence parameters - are calculated. Images of F₀, F_M, F_V, F₀', F_M', F_V', NPQ, Φ_PSII, F_V/F_M, F_V'/F_M', R_Fd, q_N, q_P, and of other fluorescence parameters are used, for example, to investigate heterogeneity due to infection, senescence, abiotic stress, or mutation.

Measured objects can be as small as isolated chloroplasts or individual cells in the Micro-FluorCam and Fluorescence Kinetic Microscope or as large as small canopies in the Arch, Transect, or Rover FluorCams. In addition to standard imaging of chlorophyll fluorescence emission, one can use the FluorCam to image fluorescence emission of all varieties of fluorescent proteins - Handy GFPCam or Closed GFPCam.